辽宁中医杂志2025，Vol.52Issue(9)：171-175,后插10,6.DOI:10.13192/j.issn.1000-1719.2025.09.044
基于"清法"理论探讨垂黄清脉饮抑制经皮腔内血管成形术术后再狭窄作用
Effect of Chuihuang Qingmai Decoction(垂黄清脉饮)on Restenosis after PTA Based on Theory of "Clearing Method"
摘要
Abstract
Objective To observe the effect of Chuihuang Qingmai Decoction(垂黄清脉饮)on anti-inflammation,inhibition of abnormal proliferation of smooth muscle cells and preventing thrombosis in restenosis model rabbits,and then to explore its mechanism of action in treating restenosis.Methods Fifty-six New Zealand rabbits were randomly divided into blank group,mod-el group,atorvastatin calcium group,Chuihuang Qingmai Decoction low-dose,medium-dose and high-dose groups,and tradi-tional Chinese and western medicine combination group.After the completion of modeling,the rabbits were administered by gavage for 4 weeks.Four weeks later,the blood was collected from the auricular vein of rabbits,and the serum levels of interleukin-6(IL-6)and tumor necorsis factor-α(TNF-α)were detected by enzyme-linked immunosorbent assay(ELISA),and the ex-pressions of the femoral artery proteins peroxisome proliferator-activated receptor γ(PPARγ)and plasminogen activator inhibitor-1(PAI1)were determined by immunohistochemistry,Western Blotting(WB)and reverse transcription-polymerase chain reaction(RT-PCR)methods.HE staining was used to observe the effect of model making and the changes of treatment.Results Com-pared with those in the blank group,the serum levels of IL-6 and TNF-α in the model group were significantly increased(P＜0.001),the expressions of femoral artery protein PPARγ and PAI1 were different(P＜0.05).Compared with those in the model group,with the increase of infusion dose,the levels of IL-6,TNF-α and PAI1 in Chuihuang Qingmai Decoction groups showed inverse ratio,and PPARγ showed positive ratio,and the changes in Chuihuang Qingmai Decoction medium dose combined with atorvastatin calcium group were significant(P＜0.001).Compared with that in the atorvastatin calcium group,the effect difference between Chuihuang Qingmai Decoction low dose group and Chuihuang Qingmai Decoction medium dose group was limited,while the difference between Chuihuang Qingmai Decoction high dose group and combination group was significant(P＜0.001).Com-pared with the medium dose group,the combined Chinese and Western medicine group had significant differences(P＜0.001).Conclusion Chuihuang Qingmai Decoction can prevent restenosis by anti-inflammation,inhibiting abnormal proliferation of smooth muscle cells and preventing thrombosis,and has a synergistic effect with atorvastatin calcium,the mechanism of which may be related to the inhibition of IL-6,TNF-α and PAI1 and activation of PPARγ.关键词
再狭窄/垂黄清脉饮/PPARγ/PAI1/平滑肌细胞增殖Key words
restenosis/Chuihuang Qingmai Decoction(垂黄清脉饮)/PPARγ/PAI1/smooth muscle cell proliferation分类
医药卫生引用本文复制引用
何霄,金学连,贾海博,王国为,赵凯..基于"清法"理论探讨垂黄清脉饮抑制经皮腔内血管成形术术后再狭窄作用[J].辽宁中医杂志,2025,52(9):171-175,后插10,6.基金项目
国家自然科学基金项目(81860845) （81860845）
中国中医科学院中医基础理论研究所合作单位个人项目(YZ-202129) （YZ-202129）
