岭南现代临床外科2025，Vol.25Issue(4)：232-238,7.DOI:10.3969/j.issn.1009-976X.2025.04.004
TGFβ1基因介导"健肾壮骨汤"调控成骨细胞Wnt3a/β-catenin信号通路
Jianshen Zhuanggu Tang targets TGF β Gene in regulation of Wnt3a/β-catenin signaling path-way on osteoblasts
摘要
Abstract
Objective To investigate the mechanism and effect of TGFβ1-mediated Jianshen Zhuanggu Tang(JSZGT)in regulating the Wnt3a/β-catenin signaling pathway on osteoblasts.Methods Osteoblast cell lines C518 and MC3T3-E1 were co-incubated with JSZGT and DKK1(an inhibitor of the Wnt3a/β-catenin signaling pathway).The differences in mRNA and protein levels of Wnt3a,TGFβ1,OPG,and RANKL in the cells were detected,and rescue experiments and RNA-seq analysis were conducted.Results After treating C518 and MC3T3-E1 cells with DKK1,the expression levels of TGFβ1,OPG,and RANKL were significantly downregulated(P<0.05).DKK1 significantly reduced the mRNA and protein levels of Wnt3a,TGFβ1,OPG,and RANKL in both the sh TGFβ1 group and the TGFβ1 over-expression(TGFβ1OE)group(P<0.05).In the C518 cell model,the knocked down expression of OPG gene inhibited the promoting effect of TGF β 1 on OPG expression;however,JSZGT treatment could restore this promoting effect of TGFβ1 on OPG expression.RNA-Seq results showed that TGFβ1,OPG,and RANKL were significantly differentially expressed genes.Conclusion The TGFβ1 gene mediates JSZGT to regulate the Wnt3a/β-catenin signaling pathway and thus modulate the expression of OPG/RANKL.The TGFβ1 gene may be an important target for the treatment of osteoporosis.关键词
TGFβ1基因/"健肾壮骨汤"/Wnt3a/β-catenin信号通路Key words
TGFβ1/Jianshen Zhuanggu Tang/Wnt3a/β-catenin signaling pathway分类
医药卫生引用本文复制引用
张镇锋,陈健聪,张勋,王国亮..TGFβ1基因介导"健肾壮骨汤"调控成骨细胞Wnt3a/β-catenin信号通路[J].岭南现代临床外科,2025,25(4):232-238,7.基金项目
广东省中医药局科研项目(编号:20231250) （编号:20231250）
