菌物学报2025，Vol.44Issue(9)：47-59,13.DOI:10.13346/j.mycosystema.250052
薄壳山核桃果生刺盘孢的遗传多样性
Genetic diversity of Colletotrichum fructicola in Carya illinoinensis
摘要
Abstract
The population genetic structure of the anthracnose fungus Colletotrichum fructicola on Carya illinoinensis was investigated based on infected samples collected from 18 production areas across 9 provinces in China.In total,78 strains of C.fructicola were identified.According to the phylogenetic analysis based on the ITS-ACT-CAL-CHS-1-GAPDH-TUB2 multi-gene system,the number of combined conserved loci in the multi-gene sequences was 1 814,with a total of 16 polymorphic loci resulting in 18 haplotypes.Five populations,including those of Songyang(ZJSY)and Xiaoshan(ZJXS)from Zhejiang Province,and Tai'an(SDTA)from Shandong Province,showed the highest haplotype diversity index(Hd=1.0),whereas population of Yuxi(YNYX)from Yunnan Province exhibited the lowest genetic diversity(Hd=0)and was significantly differentiated from other populations.Analysis of the haplotype network structure shows that there are 4 shared haplotypes and 14 exclusive haplotypes,suggesting that Hap4 is the ancestral haplotype,with 5 evolutionary branches derived from Hap4 as the center.According to the neutrality test of the population,all three values of the neutrality test were significantly negative,confirming the presence of population expansion among populations in different regions.Research shows that the C.fructicola population possesses rich genetic diversity,with gene exchange and population expansion occurring among different regions.Its genetic distance is related to geographical distribution.This study provides a foundation for deeper understanding of C.fructicola biological traits and evolutionary mechanisms.关键词
薄壳山核桃/果生刺盘孢/遗传多样性/单倍型Key words
Carya illinoensis/Colletotrichum fructicola/genetic diversity/haplotype引用本文复制引用
刘耀辉,王迪,胡云木琪儿,舒金平,翟凤艳,张亚波..薄壳山核桃果生刺盘孢的遗传多样性[J].菌物学报,2025,44(9):47-59,13.基金项目
浙江省科技计划项目(2023C02034) （2023C02034）
河南省科技攻关计划项目(232102110072) This work was supported by the Science and Technology Project of Zhejiang Province(2023C02034)and the Henan Province Science and Technology Key Research Project(232102110072). （232102110072）
