菌物学报2025，Vol.44Issue(9)：60-78,19.DOI:10.13346/j.mycosystema.250072
细脚虫草Cordyceps tenuipes线粒体基因组系统发育分析
Phylogenetic analysis of the mitochondrial genome of the Cordyceps tenuipes strains
摘要
Abstract
Cordyceps tenuipes is one of the entomogenous fungi with medicinal value.Sequencing,assembly,and annotation of the mitochondrial genomes of Cordyceps tenuipes strains YFCCYY01(Kunming,China),YFCCTL09(Chiang Mai,Thailand),and YFCCDT06(Lintong,Vietnam),revealed that the mitochondrial genomes of these three strains were circular molecules,with sizes of 40 752 bp,48 187 bp,and 32 385 bp,respectively.In total,42 genes were encoded,including 15 PCGs,2 rRNAs and 25 tRNAs.AUG was start codon and UAA was stop codon of 13 PCGs,and AUG was start codon and UAG was stop codon of nad3 and rps3;the preferred codon was UUA.The use frequency of amino acid in PCGs varies greatly,and Leu was the highest.25 tRNAs genes transport all 20 amino acids,and the secondary structure of 20 tRNA genes was cloverleaf structure.Constructing the phylogenetic tree for 51 species from six families within Cordycipitaceae,revealed that Cordyceps tenuipes belonged to the family Cordycipitaceae under the order Hypocreales.The collinearity analysis of the mitochondrial genome of Cordycipitaceae showed that there were 4 homologous regions in 14 species,and the length of the homologous region A was the longest.Compared with other Cordycipitaceae species,the mitochondrial genome of Cordyceps tenuipes is larger and the homologous region is relatively long.By investigating the mitochondrial genome of Cordyceps tenuipes,we can ascertain its phylogenetic position and characterize its species-specific features.Analysis of the differences of mitochondrial genome in different geographical locations could provide basis for the subsequent development and utilization of Cordyceps tenuipes.关键词
细脚虫草/虫草科/线粒体基因组/系统发育分析Key words
Cordyceps tenuipes/Cordycipitaceae/mitochondrial genome/phylogenetic analysis引用本文复制引用
李松宇,陆莹玲,尹瑜璠,李丹丹,虞泓..细脚虫草Cordyceps tenuipes线粒体基因组系统发育分析[J].菌物学报,2025,44(9):60-78,19.基金项目
国家自然科学基金(31870017) （31870017）
云南大学研究生科研创新基金 This work was supported by the National Natural Science Foundation of China(31870017)and the Yunnan University Postgraduate Research Innovation Fund. （31870017）
