菌物学报2025，Vol.44Issue(9)：93-104,12.DOI:10.13346/j.mycosystema.250064
香菇长链酰基辅酶A合成酶基因Le ACSL在菌种退化的作用
Role of the long chain acyl CoA synthetase gene Le ACSL in strain degeneration of Lentinula edodes
摘要
Abstract
Strain degeneration poses a significant challenge to the cultivation of edible fungi,however,the molecular mechanisms driving strain degeneration in these organisms remain relatively underexplored.In the present study,RNA interference technology was utilized to silence the long-chain acyl-CoA synthetase gene Le ACSL in Lentinula edodes.This approach successfully produced transformants exhibiting a 70%-90%reduction in gene expression.Examination of these transformants'growth on agar plates and within spawn bags revealed a 30%-40%decrease in growth rate,with colonies displaying varying degrees of sectoring and a stable phenotype.Notably,the transformants were unable to progress to the browning stage of mycelial film formation in spawn bags,leading to mycelial recession and inability to produce fruiting bodies.Microscopic analysis revealed increased branching and intertwining of the transformant mycelium,accompanied by severely wrinkled surfaces.Certain cells were observed to be entirely vacuolated and lysed,with organelles displaying compromised structural integrity.Subsequent analyses of fatty acid composition and malondialdehyde levels in the transformants revealed a 18%-29%reduction in the degree of fatty acid unsaturation and a 36%-54%decrease in lipid peroxidation compared to the wild type.These findings preliminarily suggest that the Le ACSL gene is critically involved in the strain degeneration of L.edodes.关键词
香菇/菌种退化/长链酰基辅酶A合成酶/脂肪酸/RNAiKey words
Lentinula edodes/strain degeneration/long chain acyl CoA synthetase/fatty acid/RNAi引用本文复制引用
董晶晶,吴燕莎,张丹,于海龙,宋晓霞,章炉军,谭琦,付阳,尚晓冬..香菇长链酰基辅酶A合成酶基因Le ACSL在菌种退化的作用[J].菌物学报,2025,44(9):93-104,12.基金项目
国家重点研发计划(2023YFD1201600) （2023YFD1201600）
国家自然科学基金(32472808) （32472808）
上海市农业科学院"卓越团队"建设计划([2022]001) This work was supported by the National Key Research and Development Program of China(2023YFD1201600),the National Natural Science Foundation of China(32472808),and the SAAS Program for Excellent Research Team([2022]001). （[2022]001）
