菌物学报2025，Vol.44Issue(9)：127-137,11.DOI:10.13346/j.mycosystema.250051
山东灵芝Ganoderma shandongense中的肽类化合物及其α-葡萄糖苷酶抑制活性
Peptide compounds from Ganoderma shandongense and their α-glucosidase inhibitory activities
摘要
Abstract
The peptide compounds from the rice solid-state fermentation products of Ganoderma shandongense and their α-glucosidase inhibitory activity were investigated.Ten peptide compounds were obtained through a series of separation and purification steps,including silica gel column chromatography,reverse-phase ODS column chromatography,HP20 column chromatography,and high-performance liquid chromatography(HPLC).Structural identification was carried out using mass spectrometry(MS)and nuclear magnetic resonance(NMR)spectroscopy.These compounds are:(3S,6R)-3-(cyclopropylmethyl)-6-(1H-indol-3-ylmethyl)-2,5-piperazinedione(1),cyclo-(Leu-Val)(2),cyclo-(L-Pro-L-Ile)(3),cyclo-(L-Phe-L-Pro)(4),cyclo-(Asn-phe)(5),cyclo-(L-Phe-L-Leu)(6),cyclo-(Tyr-Leu)(7),cyclo-(D-Phe-L-Ile)(8),fenestin A(9),and cyclo-(Leu-Pro-Ile-Pro)(10).The α-glucosidase inhibitory activity of compounds 1-10 was evaluated using the pNPG method.It was clear that compound 8 exhibited significant inhibitory activity with an IC50 value of(1.13±0.10)μmol/L,which was comparable to the positive control acarbose[IC50=(0.98±0.03)μmol/L].These findings provide evidence of the medicinal potential of G.shandongense.关键词
山东灵芝/化学成分/肽类/结构鉴定/α-葡萄糖苷酶抑制活性Key words
Ganoderma shandongense/chemical constituent/peptide/structural identification/α-glucosidase inhibitory activity引用本文复制引用
顾文迪,马世元,王雨曦,魏玉莲,袁海生..山东灵芝Ganoderma shandongense中的肽类化合物及其α-葡萄糖苷酶抑制活性[J].菌物学报,2025,44(9):127-137,11.基金项目
国家自然科学基金(32100013) （32100013）
辽宁省联合基金项目博士科研启动项目(2023-BSBA-328) （2023-BSBA-328）
中国科学院沈阳应用生态研究所森林生态与保育重点实验室项目(KLFES-2028) This work was supported by the National Natural Science Foundation of China(32100013),the Liaoning Provincial Joint Fund Program—Doctoral Research Initiation Project(2023-BSBA-328),and the Fund of Key Laboratory of Forest Ecology and Silviculture,Institute of Applied Ecology,Chinese Academy of Sciences(KLFES-2028). （KLFES-2028）
