菌物学报2025，Vol.44Issue(9)：138-162,25.DOI:10.13346/j.mycosystema.250079
粗毛纤孔菌乙酸乙酯提取物及活性成分的抗氧化作用机理
Antioxidant effects and functional mechanism of ethyl acetate extract and its active components of Inonotus hispidus
摘要
Abstract
The in vitro antioxidant effects and mechanisms in protecting human skin fibroblasts(HSF)from oxidative stress damage of the ethyl acetate extract of Inonotus hispidus(EAE)and its main components(fractions Fr1-Fr5 and hispidin)are investigated.Through UPLC-MS detection,in vitro antioxidant experiments,network pharmacology,and other experimental methods,the composition of polyphenolic compounds in EAE,the reducing ability and free radical scavenging ability of EAE,Fr1-Fr5,and hispidin,as well as the potential targets and pathways of hispidin in treating oxidative stress diseases were investigated.Result indicates that EAE contains various polyphenolic compounds,with hispidin being the primary one.EAE,Fr1-Fr5,and hispidin all exhibit certain reducing and free radical scavenging abilities,with antioxidant capacity ranking as EAE>Fr1-Fr5>hispidin.Network pharmacology analysis speculated that hispidin had nine core targets for treating oxidative stress diseases and they were mainly enriched in the MAPK signaling pathway and several other signaling pathways.An oxidative stress injury model for HSF cells was established.By using various methods,cell viability,reactive oxygen species(ROS)levels,collagen levels,and the expression of related proteins and relative messenger RNA(mRNA)were detected.The results indicate that 200 μg/mL of EAE,Fr4,and hispidin can enhance the survival rate and antioxidant capacity of HSF under oxidative stress,reduce malondialdehyde(MDA)content and ROS levels,promote the synthesis of type Ⅰ collagen(COLI),and possibly inhibit its degradation by regulating the MMPs/TIMPs system in the MAPK pathway.EAE has a significant protective effect on HSF under oxidative damage.In summary,this study verified that the ethyl acetate extract of I.hispidus and its active components possess strong antioxidant capabilities and can improve damage caused by oxidative stress by acting on multiple targets through the MAPK signaling pathway.关键词
粗毛纤孔菌/抗氧化活性/人皮肤成纤维细胞(HSF)/氧化应激Key words
Inonotus hispidus/antioxidant activity/human skin fibroblasts(HSF)/oxidative stress引用本文复制引用
吕雪,包海鹰,李玉,霍慧敏..粗毛纤孔菌乙酸乙酯提取物及活性成分的抗氧化作用机理[J].菌物学报,2025,44(9):138-162,25.基金项目
吉林农业大学校企合作项目(20210080) This work was supported by the Jilin Agricultural University School-Enterprise Cooperation Project(20210080). （20210080）
