日用化学品科学2025，Vol.48Issue(9)：56-61,6.DOI:10.3969/j.issn.1006-7264.2025.09.009
人源表皮葡萄球菌的分离鉴定和抗炎活性研究
Isolation,identification,and anti-inflammatory activity of Staphylococcus epidermidis from human skin
摘要
Abstract
To understand the intervention effect of Staphylococcus epidermidis from human skin on the skin inflammation,37 skin swab samples were collected in Guangzhou,Staphylococcus bacteria were isolated and purified by screening medium,and the isolated strains were identified by 16S rRNA sequencing to construct an phylogenetic tree.An skin inflammatory model with high expression of pyroptosis were constructed using antioxidant assays and lipopolysaccharides(LPS),and the antioxidant activity of isolated strains and their inhibitory effect on the pyroptosis pathway were evaluated.The results showed that one strain of Staphylococcus epidermidis from human skin was isolated and named M-5Y.After the fermentation,the M-5Y fermentation supernatant can significantly scavenge DPPH and ABTS free radicals,and has good antioxidant properties.Meanwhile,the M-5Y fermentation supernatant can significantly inhibit LPS activated pyroptosis pathway in BJ cells,which demonstrates the significant anti-inflammatory properties.关键词
表皮葡萄球菌/抗炎/抗氧/皮肤Key words
Staphylococcus epidermidis/anti-inflammatory/antioxidant/skin分类
化学化工引用本文复制引用
叶林,吴铭杰,聂艳峰,郭朝万,裴运林,吴绮婷,张昀..人源表皮葡萄球菌的分离鉴定和抗炎活性研究[J].日用化学品科学,2025,48(9):56-61,6.基金项目
高端日用化学品绿色生物制造关键技术及产业化应用(2022B1111080003) （2022B1111080003）
