山东农业科学2025，Vol.57Issue(8)：24-34,11.DOI:10.14083/j.issn.1001-4942.2025.08.004
'金如意'山楂叶绿体基因组特征与系统进化分析
Characterization and Phylogenetic Analysis of Complete Chloroplast Genome of Crataegus pinnatifida Bge.var.major Jinruyi
摘要
Abstract
Using the leaves of Crataegus pinnatifida Bge.var.major Jinruyi as materials,the complete chloroplast genome sequence was sequenced,assembled and analyzed by Illumina high-throughput sequencing technology in this study.The phylogenetic analysis was also conducted on the chloroplast genomes of 34 Rosa-ceae species.The results showed that the complete chloroplast genome of C.pinnatifida Bge.var.major Jinruyi was 159 655 bp in length with the GC content of 36.65％,and contained a large single copy(LSC)region of 87 748 bp,a small single copy(SSC)region of 19 139 bp and a pair of inverted repeats(IR)regions of 26 384 bp.A total of 130 genes were encoded,including 85 protein-coding genes,37 tRNA genes,and 8 rRNA genes.A total of 264 SSR loci and 57 repeats were detected by repeat analysis with most SSR loci con-sisting of A-and T-repeat bases.There were 31 high-frequency codons(RSCU＞1),29 of which ended with A/U.Compared with other Rosaceae species,the IR region boundary of the C.pinnatifida Bge.var.major Jin-ruyi chloroplast genome was relatively conserved.The phylogenetic analysis showed that C.pinnatifida Bge.var.major Jinruyi was most closely related to C.pinnatifida var.major(GenBank No.NC_065486.1),indica-ting that it belonged to the large-fruit hawhorn type.These findings could lay a foundation for the identification of hawthorn germplasm resources,the analysis of genetic diversity,and the utilization of hawthorn resources.关键词
'金如意'山楂/叶绿体基因组/系统进化分析Key words
Crataegus pinnatifida Bge.var.major Jinruyi/Chloroplast genome/Phylogenetic analysis分类
农业科技引用本文复制引用
林冉冉,王宏伟,焦慧君,董肖昌,关秋竹,魏树伟,聂宗省,张玮玮,冉昆..'金如意'山楂叶绿体基因组特征与系统进化分析[J].山东农业科学,2025,57(8):24-34,11.基金项目
山东省重点研发计划(农业良种工程)项目(2024LZGC029) （农业良种工程）
山东省重点研发计划(乡村振兴科技创新提振行动计划)项目(2024TZXD041) （乡村振兴科技创新提振行动计划）
科技基础资源调查专项专题任务"华东地区山楂野生优特异种质资源调查"(2024FY100502-7) （2024FY100502-7）
泰安市农业良种工程项目(2025NYLZ06) （2025NYLZ06）
