山东农业科学2025，Vol.57Issue(8)：66-74,9.DOI:10.14083/j.issn.1001-4942.2025.08.009
精氨酸对低温冷害下水稻幼苗形态特征及生理特性的影响
Effects of Arginine on Morphological and Physiological Characteristics of Rice Seedlings under Low Temperature Stress
摘要
Abstract
This experiment used Oryza sativa'Xiangzaoxian 45'as test material and conducted under the artificially simulated low-temperature(4 ℃)environment.With spraying distilled water followed by nor-mal-temperature(25 ℃)treatment(CK1)and low-temperature(4 ℃)treatment(CK2)as control groups,spraying arginine solutions at different concentrations of 0.025,0.25,2.5 and 25 mmol/L(labeled as T1,T2,T3 and T4,respectively)were set to study their effects on the morphological and physiological character-istics of rice seedlings under low-temperature stress at 12,24 and 36 hours.The results demonstrated that low-temperature stress significantly inhibited plant height,aboveground fresh weight and chlorophyll biosynthesis in leaves.However,appropriate arginine concentrations effectively alleviated these inhibitions.After 12 hours low-temperature treatment,T2(0.25 mmol/L arginine)significantly increased plant height by 6.73％com-pared to CK2,and also increased the total root length,root surface area,root tip number and root branching number by 2.84％,10.06％,3.30％and 4.20％,respectively.After 24 hours low-temperature stress,T2 sig-nificantly enhanced plant height and stem diameter by 15.85％and 6.73％,respectively.After 36 hours of low-temperature treatment,T2 increased the aboveground fresh weight by 10.16％compared with CK2.After 12 hours and 24 hours low-temperature exposure,T2 significantly increased the chlorophyll b and total chlorophyll levels by 59.13％,31.74％and 94.43％,29.95％,respectively,compared to CK2.Arginine application en-hanced the peroxidase(POD)and catalase(CAT)activities in both leaves and roots under low-temperature stress.After 24 hours treatment,the activities of SOD and POD in leaves treated with T2 were significantly higher than those of CK2,with the increase rates as 29.69％and 30.53％,respectively.In sum,spraying 0.25 mmol/L arginine effectively alleviated the adverse effects of low-temperature stress on rice seedling morphologi-cal development and physiological activity,thus enhanced the cold resistance during seedling stage.关键词
水稻幼苗/精氨酸/低温胁迫/形态特征/生理特性Key words
Rice seedling/Arginine/Low-temperature stress/Morphological characteristics/Physiologi-cal characteristics分类
农业科技引用本文复制引用
周露,熊佳铭,刘玲,曾红丽,屈成,潘素君..精氨酸对低温冷害下水稻幼苗形态特征及生理特性的影响[J].山东农业科学,2025,57(8):66-74,9.基金项目
国家重点研发计划项目(2023YFD2301404) （2023YFD2301404）
中国烟草总公司湖南省公司科技项目(HN2023KJ03) （HN2023KJ03）
