药学研究2025，Vol.44Issue(8)：750-755,6.DOI:10.13506/j.cnki.jpr.2025.08.004
黄芩苷对小鼠急性肝损伤的作用及机制研究
Effect and mechanism of baicalin on acute liver injury in mice
摘要
Abstract
Objective To study the effect and mechanism of baicalin on acute liver injury(ALI)in mice induced by carbon tetrachloride(CCl4).Methods Male C57BL/6JNifdc mice were randomly divided into normal group,model group,positive drug group,and baicalin low,medium,and high dose groups.The ALI model of mice was prepared by intraperitoneal injection of 0.1％CCl4 peanut oil solution.Each group was given orally for 7 days.The mouse NCTC1469 cells were randomly divided into normal group,model group,baicalin group,TAK-242 group and baicalin+TAK-242 group.The in vitro ALI model was established with CCl4 of the final concentration of 10 mmol·L-1.The contents of alanine aminotransferase(ALT),aspartate aminotransferase(AST)and alkaline phosphatase(ALP)in serum and the contents of interleukin-1β(IL-1β),IL-6 and tumor necrosis factor-α(TNF-α)in serum and cell supernatant were determined.The protein expressions of Toll-like receptor 4(TLR4),inhibitor-κB kinase ε(IKKε)and tank-binding kinase 1(TBK1)were detected in liver tissues and cells.Results The contents of ALT,AST and ALP,IL-1β,IL-6 and TNF-α in serum of ALI mice,and the expression of TLR4,TBK1 and IKKε in liver tissue were significantly increased compared with the normal group.Besides,liver cell necrosis,inflammatory cell infiltration and hepatic cord arrangement disorder were observed in liver of ALI mice.Baicalin could significantly reverse the abnormal expression of the above indexes;In cell experiments,it was found that CCl4 induced significantly increased contents of IL-1β,IL-6 and TNF-α in the supernatant of NCTC1469 cells,as well as the expression of TLR4,TBK1 and IKKε proteins in the cells.Baicalin,TAK-242 and baicalin+TAK-242 significantly decreased the contents of IL-1β,IL-6 and TNF-α as well as the expression of TLR4,TBK1 and IKKε in the cells,and there was no significant difference in the above indexes in TAK-242 and baicalin+TAK-242 groups.Conclusion Baicalin may regulate inflammation and improve CCl4-induced ALI through TLR4-TBK1-IKKε axis.关键词
急性肝损伤/黄芩苷/炎症/Toll样受体4-TANK结合激酶1-κB抑制因子激酸εKey words
Acute liver injury/Baicalin/Inflammation/TLR4-TBK1-IKKε axis分类
医药卫生引用本文复制引用
邹祥,毛占存,王星..黄芩苷对小鼠急性肝损伤的作用及机制研究[J].药学研究,2025,44(8):750-755,6.基金项目
江苏省基础研究计划(自然科学基金)-青年基金项目(No.BK20180574) （自然科学基金）
2021年句容市社会发展科技计划项目(No.ZA42104) （No.ZA42104）
