水生态学杂志2025，Vol.46Issue(5)：258-268,11.DOI:10.15928/j.1674-3075.202409050001
鸭绿江口湿地土壤微生物群落结构与碳循环的宏基因组学
Macrogenomic Study of the Soil Microbial Community Structure and Carbon Cycle in Yalu River Estuary Wetland
摘要
Abstract
Exploring the structure of the wetland microbial community is important because it can reveal the relationship between microbial activity and wetland ecosystem function.In this study,the Yalu River estuary wetland was selected for research,and we investigated microbial community composition,diversi-ty,and carbon cycle functional genes within five soil types(tidal flat saline soil,bog soil,paddy soil,meadow soil,and brown forest soil)using metagenomic sequencing technology.Additionally,we exam-ined the impact of soil physiochemical factors on microbial community structure and carbon cycling path-ways.We aimed to provide a scientific foundation for the rational development,restoration,and protec-tion of wetland ecological functions.On September 29 of 2022,surface soil samples of each soil type(0-20cm)were collected from five sampling sites in the wetland.At each site,three sampling points were ran-domly selected using a five-point sampling method.The soil samples from each site were thoroughly mixed and then divided into two portions,one for DNA extraction and high-throughput sequencing,and the other for analysis of soil physicochemical properties.Among the soil types,there was no significant difference in the Chaol index for the bacterial and fungal communities(P＞0.05),but there was a signifi-cant difference in the Shannon index(P＜0.05).For the archaea communities,both Chao1 and Shannon in-dices were significantly different(P＜0.05).The primary bacterial phyla were Proteobacteria,Actinobacte-ria,Acidobacteria,Bacteroidetes,and Nitrospirae.The Archaea phyla were dominated by Thaumarchaeo-ta,Crenarchaeota,and Euryarchaeota.Ascomycota,Mucoromycota,and Basidiomycota were the most dominant fungal phyla.Redundancy analysis(RDA)between the microbial community and environmen-tal factors showed that soil organic carbon,total phosphorus,electrical conductivity,and pH were the pri-mary influencing factors of bacterial communities,while total phosphorus,total sulfur,and electrical con-ductivity were the primary influencing factors for archaeal communities.For fungal communities,the key driving factors were electrical conductivity,total phosphorus,total sulfur,and total nitrogen.Based on the DiTing database,29 carbon pathways involving 114 gene families were detected,with variations among soil types and the gene abundance associated with each functional cycle pathway.The WL(Wood-Ljung-dahl pathway)carbon fixation pathway and methanogenesis with methanol and acetic acid as substrates,occurred only in bog soil.Tidal flat saline soil contributed the most to the reverse tricarboxylic acid cycle(rTCA)and methane metabolism,while paddy soil contributed the most to the CO2-dependent methano-genesis pathway.The Mantel test results show that pH,total nitrogen,soil organic carbon,and total phos-phorus were the primary influencing factors for soil microbial carbon cycling genes.Sankey diagram anal-ysis revealed that Proteobacteriota,Euryarchaeota,Thaumarchaeota,Actinomyceotota,and Bacteroidota,along with their corresponding 18 genera,were the primary contributors to carbon cycling in the wetland.This study provides an important reference for the protection and management of the wetland ecosystem in Yalu River estuary.关键词
宏基因组/微生物群落结构/碳循环/鸭绿江口湿地Key words
metagenome/microbial community structure/carbon cycling/Yalu River estuary wetland分类
生物科学引用本文复制引用
宋宇,高明,张春鹏,曲贵伟,李富祥..鸭绿江口湿地土壤微生物群落结构与碳循环的宏基因组学[J].水生态学杂志,2025,46(5):258-268,11.基金项目
辽宁省教育厅项目(JYTMS20230701) （JYTMS20230701）
辽东学院鸭绿江流域研究院开放课题(YLJ20230105) （YLJ20230105）
辽宁省属本科高校基本科研业务费专项资金(2024TD004) （2024TD004）
鸭绿江流域生物多样性科研平台建设(LJ232411779041). （LJ232411779041）
