世界科学技术-中医药现代化2025，Vol.27Issue(9)：2503-2512,10.DOI:10.11842/wst.20241128002
基于TLR4/Myd88/NF-κB信号通路探讨瑶医水莲风亏方对盆腔炎性疾病后遗症大鼠的改善作用
Protective Effects of Yao Medicine Shuilian Fengkui Formula on Sequelae of Pelvic Inflammatory Disease in Rats Through Inhibiting TLR4/Myd88/NF-κB Signaling Pathway
摘要
Abstract
Objective To investigate the protective effects and mechanism of Shuilian Fengkui Formula(SFF)of Yao medicine on sequelae pelvic inflammatory disease(SPID)in rats.Methods The SPID rat model was established by mechanical injury coupled with bacterial infection,and then the rats were randomly divided into:the SPID group,the positive drug(Fukeqianjin tablets)group,SFF groups at low,medium and high doses,and the sham-operation group,with 8 rats in each group;SPID group,SFF groups,TLR4 agonist CRX-527(CRX-527)group,SFF plus CRX-527 group,with 8 rats in each group.Corresponding drug intervention was given for 21 consecutive days.The indexes of uterus,spleen,thymus and other organs were calculated.The histopathological changes in rat uterus were observed by HE staining.The levels of interleukin-1β(IL-1β),interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),immunoglobulin G(IgG),immunoglobulin M(IgM)in serum were detected by ELISA;The protein expression levels of Toll receptor 4(TLR4),myeloid differentiation factor(MyD88),nuclear factor kappa B p65(NF-κB p65),and phosphorylated nuclear factor kappa B p65(p-NF-κB p65)in rat uterine tissues were detected by Western blot.Results Compared with the sham operation group,the uterine index of SPID group significantly increased(P<0.01),the spleen index and thymus index significantly decreased(P<0.01),the pathological damage of uterine tissue was serious,the contents of IL-1β,IL-6,TNF-α in serum significantly increased(P<0.01),the contents of IgG and IgM significantly decreased(P<0.01),the TLR4,MyD88 protein and the ratio of p-NF-κB p65/NF-κB p65 protein in uterine tissue significantly increased(P<0.01).Compared with the SPID group,the uterine index of the positive drug group and the SFF groups significantly decreased(P<0.05),the spleen index and thymus index significantly increased(P<0.05),the pathological damage of uterine tissue significantly improved,the contents of IL-1β,IL-6 and TNF-α in serum were significantly lowered(P<0.05),the contents of IgG and IgM significantly increased(P<0.05),and the TLR4,MyD88 protein and the ratio of p-NF-κB p65/NF-κB p65 protein in uterine tissue significantly downregulated(P<0.01).However,CRX-527 significantly reversed the effects of SFF on SPID in rats.Conclusion SFF could improve SPID in rats by impeding the inflammatory response,and its mechanism might be attributed to inhibition of TLR4/MyD88/NF-κB signaling pathway.关键词
盆腔炎性疾病后遗症/瑶医/水莲风亏方/TLR4/MyD88/NF-κB信号通路/炎症Key words
Sequelae of pelvic inflammatory disease/Yao medicine/Shuilian Fengkui formula/TLR4/MyD88/NF-κB signaling pathway/Inflammation分类
医药卫生引用本文复制引用
覃倩,吕超,刘莉,崔佳瑜,陈姗,徐航..基于TLR4/Myd88/NF-κB信号通路探讨瑶医水莲风亏方对盆腔炎性疾病后遗症大鼠的改善作用[J].世界科学技术-中医药现代化,2025,27(9):2503-2512,10.基金项目
广西自然科学基金青年科学项目(2023GXNSFBA026062):基于TLR4/Myd88通路探讨瑶医水莲风亏方影响子宫内膜异位症的应用基础研究,负责人:覃倩. （2023GXNSFBA026062）
