色谱2025，Vol.43Issue(10)：1177-1183,7.DOI:10.3724/SP.J.1123.2024.11024
本科仪器分析实验设计与实践:固相微萃取-液相色谱检测环境水体中的多环芳烃
Design and practice of undergraduate instrumental analysis experiment:analysis of polycyclic aromatic hydrocarbons in environmental water by solid-phase microextraction-liquid chromatography
摘要
Abstract
A research-project-based open instrumental-analysis experiment was designed for un-dergraduate teaching led by the attributes of an environmental science major.In this experiment,porous monolithic fibers are in-situ synthesized from 9-vinylanthracene and styrene(as a monomer mixture)based on the molecular characteristics of studied polycyclic aromatic hydrocarbons(PAHs),and then used as an extractant for solid-phase microextraction(SPME).A method for reliably monitoring PAHs in environmental water samples was developed by combining SPME with high performance liquid chromatography(HPLC).Students designed and constructed a complete experimental instrumental-analysis process,which included preparing and characterizing the monolith fibers,optimizing the SPME conditions,preparing environmental water samples,and analysis by HPLC.Guided by teachers,undergraduates learned relevant knowledge prior to per-forming the experiment,improved their operating skills during the experiment,analyzed data,and wrote research reports during the latter stage of the course.Undergraduates'interest is stimulated by completing the experiment,while scientific thinking,analysis,and problem-solving abilities are concurrently improved and cultivated.The present course also lays the foundation for cultivating future high-quality environmental-science talents.关键词
本科实验教学/固相微萃取/仪器分析/多环芳烃/液相色谱Key words
undergraduate experimental teaching/solid-phase microextraction(SPME)/instru-mental analysis/polycyclic aromatic hydrocarbons(PAHs)/liquid chromatography(LC)分类
化学化工引用本文复制引用
刘珺,吴江毅,李锦涛,陈能汪,黄晓佳..本科仪器分析实验设计与实践:固相微萃取-液相色谱检测环境水体中的多环芳烃[J].色谱,2025,43(10):1177-1183,7.基金项目
福建省本科高校教育教学研究项目(FBJY20240253) （FBJY20240253）
广东省基础与应用基础研究基金(2024A1515011556) （2024A1515011556）
国家自然科学基金(22576172).Undergraduate Education and Teaching Research Project in Fujian Province(No.FBJY20240253) （22576172）
Guangdong Basic and Applied Basic Research Foundation(No.2024A1515011556) （No.2024A1515011556）
National Natural Sci-ence Foundation of China(No.22576172). （No.22576172）
