生物技术通报2025，Vol.41Issue(9)：22-31,10.DOI:10.13560/j.cnki.biotech.bull.1985.2025-0276
药用植物空间代谢组学研究进展
Advances in Spatial Metabolomics in Medicinal Plants
摘要
Abstract
Mass spectrometry imaging(MSI)is an innovative molecular imaging technique offering significant advantages,including label-free analysis,high molecular coverage,and exceptional sensitivity.MSI has been widely used in studies on the spatial distribution of metabolites in tissues.The continuous advancement of this technique has brought spatial metabolomics,a methodology that integrates MSI with metabolomics.This approach allows for simultaneous localization and imaging analysis of metabolites in tissues across diverse biological samples,encompassing animals,plants,and microorganisms.Recently,spatial metabolomics has demonstrated great potential in medicinal plant research as a crucial technique for precise spatial localization of metabolites in tissues.This review first presents the basic principles and experimental procedures of spatial metabolomics,followed by comparisons of several major MSI techniques,including matrix-assisted laser desorption/ionization mass spectrometry imaging(MALDI-MSI),desorption electrospray ionization mass spectrometry imaging(DESI-MSI),and secondary ion mass spectrometry imaging(SIMS-MSI),where distinctive advantages and limitations of each approach are delineated,as well as key aspects in sample preparation and data processing are addressed.The review subsequently focuses on the applications of spatial metabolomics in medicinal plants,including visualizing the spatial distribution and accumulation pattern of metabolites,clarifying the biosynthesis and transport sites of metabolites,and discovering functional genes associated with biosynthetic pathways in medicinal plants.Finally,this review discusses the current challenges and prospects of spatial metabolomics,aiming to provide a new perspective for investigations in medicinal plant research.关键词
空间代谢组学/质谱成像/药用植物/代谢产物/生物合成Key words
spatial metabolomics/mass spectrometry imaging/medicinal plants/metabolites/biosynthesis引用本文复制引用
刘语诗,李镇,邹宇琛,汤维维,李彬..药用植物空间代谢组学研究进展[J].生物技术通报,2025,41(9):22-31,10.基金项目
国家自然科学基金面上项目(82374028) （82374028）
