生物技术通报2025,Vol.41Issue(9):32-43,12.DOI:10.13560/j.cnki.biotech.bull.1985.2025-0397
玉米和高粱遗传转化技术研究进展
Research Progress in Genetic Transformation Technologies of Maize and Sorghum
摘要
Abstract
Plant genetic transformation technology,as a key method for crop genetic improvement,enables the stable integration of foreign functional genes to effectively regulate important traits such as plant growth and development,stress resistance,and environmental adaptability.The research on plant genetic transformation technology not only provides a key technical platform for gene function validation but also significantly promotes the establishment and development of precision crop breeding systems through the directed improvement of target traits.To achieve efficient and stable genetic transformation,researchers have been continuously optimizing and developing diverse technological approaches,primarily focusing on the effective delivery of exogenous genes and the high-efficiency regeneration of transformed plants.This review summarized the two primary methods for genetic transformation in maize(Zea mays L.)and sorghum(Sorghum bicolor(L.)Moench),the biolistic method and Agrobacterium-mediated transformation.The review highlighted critical factors affecting the efficiency of Agrobacterium-mediated transformation.And it also summarized key genes that enhance genetic transformation efficiency and their applications in plant genetic transformation.Additionally,the review summarized challenges and proposed strategies to optimize transformation systems using key regulatory genes.It also outlined the future research directions to provide insights for enhancing transformation efficiency and their applications in high-throughput molecular breeding.关键词
玉米/高粱/遗传转化/转化效率/关键基因Key words
maize/sorghum/genetic transformation/transformation efficiency/key genes引用本文复制引用
胡璐,王凯,徐婧仪,叶丽慧,王永飞,王丽华,李杰勤..玉米和高粱遗传转化技术研究进展[J].生物技术通报,2025,41(9):32-43,12.基金项目
国家自然科学基金项目(32372134),滁州市"双创之星"产业创新团队 (32372134)