生物技术通报2025，Vol.41Issue(9)：54-61,8.DOI:10.13560/j.cnki.biotech.bull.1985.2025-0351
基于BSA-seq的番木瓜株高候选基因定位
Gene Mapping of Plant Height in Papaya Based on BSA-seq
摘要
Abstract
[Objective]Plant height is one of the most important traits affecting the yield and quality of papaya(Carica papaya),and mapping the major candidate genes for papaya plant height provides genetic resources for the dwarf breeding of papaya.[Method]We used YY(taller plant)and GM(shorter plant)as the parents to construct a mapping population.The two parents and the offspring pools GY-H(taller pool)and GY-S(shorter pool)were then sequenced using BSA-seq technology.After the data were quality-controlled,the gene frequency gap between the pools was calculated by using the ED and SNP-index methods to locate the candidate intervals of the papaya plant height.[Result]The BSA-seq generated high-quality data,resulting in 337 980 SNP loci for gene mapping.The significant differences in genotype frequencies between the mixed pools were calculated using the ED and SNP-index methods,leading to the localization of candidate intervals with a size of 0.93 Mb on chromosome 3,6,and 8.The interval was compared to the'Zihui'papaya reference genome and found to contain 46 coding genes,37 of which were annotated to various databases.[Conclusion]Cp_zihui12764,Cp_zihui06963,Cp_zihui07024,and Cp_zihui12732 are likely to be candidate genes to regulate the heights of the papaya plants.关键词
番木瓜/株高/BSA-seq/基因定位/候选基因Key words
Carica papaya/plant height/BSA-seq/gene mapping/candidate gene引用本文复制引用
吴夏明,周陈平,杨敏,徐泽,邝瑞彬,刘传和,贺涵,魏岳荣..基于BSA-seq的番木瓜株高候选基因定位[J].生物技术通报,2025,41(9):54-61,8.基金项目
广州市重点研发计划(2023B03J1369),广东省农业科学院果树研究所自主培育项目(2023001),2024年广东省乡村振兴战略专项(2024-NPY-00-028) （2023B03J1369）
