生物技术通报2025，Vol.41Issue(9)：94-104,11.DOI:10.13560/j.cnki.biotech.bull.1985.2025-0142
基于SSR标记的马铃薯野生种和地方种遗传多样性分析和指纹图谱构建
Genetic Diversity Analysis and Fingerprinting of Wild Potato and Landraces Based on SSR Markers
摘要
Abstract
[Objective]Analyze genetic diversity of wild and landrace potato species at the molecular level and establish a fingerprint database for potato germplasm resources.[Method]SSR(Simple Sequence Repeat)molecular markers was used to analyze the genetic diversity of 73 wild potato and landrace potato species preserved at the CIP-China Center for Asia Pacific.[Result]The genetic diversity analysis revealed an average number of alleles(Na)of 1.843 7,Nei's gene diversity index of 0.065 9,Shannon's information index of 0.128 6,and an average polymorphism information content(PIC)value of 0.848 4,indicating high polymorphism but low genetic differentiation among the 73 germplasm resources.The germplasms were classified into seven subpopulations by Structure analysis,whereas they were divided into four groups via UPGMA clustering.[Conclusion]Five pairs of core primers are selected from 14 SSR primers,enabling complete differentiation of all 73 accessions.These primers were used to construct unique DNA fingerprints for the 73 wild and landrace potato accessions.This work provides a theoretical foundation and technical framework for future germplasm identification and screening systems in potato breeding.关键词
马铃薯/野生种/地方种/遗传多样性/SSR/指纹图谱Key words
potato/wild potato/landraces/genetic diversity/SSR(Simple Sequence Repeat)/fingerprinting引用本文复制引用
卢瑶,袁平平,金鑫,毛向红,范向斌,白小东..基于SSR标记的马铃薯野生种和地方种遗传多样性分析和指纹图谱构建[J].生物技术通报,2025,41(9):94-104,11.基金项目
山西省高等学校科技创新项目(2023L038),山西农业大学高寒区作物研究所所级项目(GHS202402),国家马铃薯产业技术体系(CARS-09-ES02),山西省现代农业产业技术体系建设专项资金资助(2025CYJSTX06),大同市应用基础研究计划项目(2024078) （2023L038）
