生物技术通报2025，Vol.41Issue(9)：159-167,9.DOI:10.13560/j.cnki.biotech.bull.1985.2025-0147
荔枝LcTFL1基因的克隆与功能分析
Cloning and Functional Analysis of LcTFL1 Gene in Litchi chinensis Sonn
摘要
Abstract
[Objective]The TERMINAL FLOWER 1(TFL1)gene is a crucial regulator in the plant flowering pathway.Our study aims to clone the LcTFL1 gene of litchi(Litchi chinensis Sonn.)and analyze its expression pattern and function,with the goal of identifying a marker gene for litchi flower formation regulation.This helps unlock the genetic mechanisms governing litchi floral development.[Method]Using previously obtained transcriptome data,we designed specific primers and successfully cloned the LcTFL1 gene from'Feizixiao'litchi leaves by using the PCR technology.To verify its function,we used bioinformatics for protein structure and phylogenetic analysis.Through quantitative PCR technology,different tissues of litchi were selected to clarify the tissue expression pattern of this gene.Subcellular localization was determined by fusing LcTFL1 with a fluorescent tag and observing with confocal microscopy.By constructing a plant dual-source expression vector,we also overexpressed LcTFL1 in Arabidopsis thaliana to study its impact on flowering.[Result]The full-length coding region of LcTFL1 is 513 bp,which encodes 170 amino acids.It is a hydrophilic protein with a conserved PEBP domain.The promoter has cis-acting elements responsive to light,hormones,and stress.LcTFL1 localizes to the cytoplasm.Phylogenetically,it is the closest to JRO89 of Xanthoceras sorbifolium.Its expression is the highest in seeds,followed by apical buds and fruits.When the LcTFL1 gene was overexpressed in A.thaliana,the overexpressed A.thaliana plants showed that the number of rosette leaves at the initial flowering stage significantly increased,the length and width of leaves significantly reduced,and the plants demonstrated a late-flowering phenotype.[Conclusion]High expression of LcTFL1 delays A.thaliana flowering,indicating it acts as an inhibitory factor in flowering regulation.This discovery has implications for understanding plant development and for crop improvement in litchi.关键词
荔枝/LcTFL1基因/表达模式/过表达/开花Key words
litchi/LcTFL1 gene/expression pattern/over-expression/flowering引用本文复制引用
史发超,姜永华,刘海伦,文英杰,严倩..荔枝LcTFL1基因的克隆与功能分析[J].生物技术通报,2025,41(9):159-167,9.基金项目
广州市科技计划项目(2023A04J0137),国家自然科学基金项目(32102333) （2023A04J0137）
