生物技术通报2025，Vol.41Issue(9)：168-181,14.DOI:10.13560/j.cnki.biotech.bull.1985.2025-0279
转录组和代谢组联合解析'桂柚1号'和'沙田柚'果实品质差异
Integrate Transcriptomic and Metabolomic Analysis of Fruits Quality Differences between'Guiyou No.1'and'Shatianyou'Pomelo(Citrus maxima)
摘要
Abstract
[Objective]This study aims to elucidate the differences of fruit qualities and metabolic regulation between the self-pollinated cultivar pomelo'Guiyou No.1'and its female parent'Shatianyou',it will provide a theoretical basis for future comprehensive research on the mechanisms of fruit quality formation and the metabolic regulation in pomelo.[Method]Based on physicochemical parameter measurements,transcriptomics,and widely targeted metabolomics,the fruit qualities,gene expression levels,and metabolite profiles were systematically analyzed in mature fruits of'Guiyou No.1'and'Shatianyou'pomelo.[Result]There were no significant differences in fruit weight,soluble solids content,titratable acidity,and vitamin C content between pomelo'Guiyou No.1'and'Shatianyou'.Fruit shape index in'Guiyou No.1'was significantly greater than that in'Shatianyou',while the total amino acid content was significantly less than that in'Shatianyou'.Compared to'Shatianyou',the 285 differentially expressed genes(DEGs)were screened,including 190 up-regulated genes and 95 down-regulated genes,respectively.These DEGs were primarily enriched in metabolic pathways,such as arginine and proline metabolism,fatty acid metabolism,and phenylpropanoid biosynthesis.Metabolome profiling suggested that a total of 90 differential metabolites were detected in'Guiyou No.1',with 17 up-regulated and 30 down-regulated ones,compared with pomelo'Shatianyou'.Upregulated metabolites were mainly alkaloids and coumarins,while downregulated ones included amino acids,flavonoids,and phenolic acids.The differences of fruit quality between'Guiyou No.1'and'Shatianyou'were primarily influenced by the pathways of amino acids,alkaloids,and phenylpropanoids.Key genes such as GOT2,COMT,UGT73C,CYP71A and PAIC were identified as potentially playing crucial roles in the quality formation of pomelo fruits.[Conclusion]No significant differences in general qualities,are detected between mature fruits of'Guiyou No.1'and'Shatianyou'pomelo;however,there are obvious differences in terms of amino acids,flavonoids,phenolic acids,and alkaloids.The differential genes and metabolites between the two cultivars are mainly enriched in the metabolic pathways of amino acid and phenylpropanoid.关键词
桂柚1号/沙田柚/果实品质/转录组/代谢组/氨基酸/类黄酮/生物碱Key words
Guiyou No.1/Shatianyou/fruit qualities/transcriptome/metabolomics/amino acids/flavonoids/alkaloids引用本文复制引用
刘建国,刘格儿,郭颖欣,王斌,王玉昆,卢金凤,黄文庭,朱云娜..转录组和代谢组联合解析'桂柚1号'和'沙田柚'果实品质差异[J].生物技术通报,2025,41(9):168-181,14.基金项目
韶关市科技计划项目(220610114530885),广东省教育厅重点科研平台和项目(2024GCZX006) （220610114530885）
