生物技术通报2025，Vol.41Issue(9)：265-276,12.DOI:10.13560/j.cnki.biotech.bull.1985.2025-0364
产Surfactin贝莱斯芽胞杆菌C5A-1的鉴定和所产Surfactin对植物的促生效果
Identification of Surfactin-producing Bacillus Velezensis C5A-1 and Evaluation of the Plant Growth-promoting Effects of Its Surfactin
摘要
Abstract
[Objective]This research is aimed to screen the high surfactin-producing strains,understand the genetic background and surfactin biosynthesis of the strains from the whole genome,and explore the functions of surfactin produced by the strains in plant growth-promoting.[Method]Surfactin-producing strains were isolated from plant rhizosphere soil by selective separation technique.The quantification and structural characterization of surfactin were performed using high-performance liquid chromatography and MALDI-TOF MS,respectively.The whole-genome was sequenced using Illumina+PacBio third-generation sequencing platform;and species identification,functional gene annotation,secondary metabolic gene clusters and surfactin biosynthesis were analyzed by bioinformatics method.Pot experiments were performed to detect the plant growth-promoting effect by surfactin.[Result]Strain C5A-1 with strong oil-displacing and antifungal activity was screened from poplar rhizosphere soil collected from Datong,Shanxi province.Surfactin homologues from C5A-1 were identified as C14-and C15-surfactin A,with the yield of 1 208.16 mg/L.The genomic average nucleotide identity(ANI)and digital DNA-DNA hybridization values between C5A-1 and B.velezensis FZB42 were 98.35%and 85.4%,respectively.C5A-1 genome size was 3 929 585 bp with 46.5%guanine-cytosine content,encoding 3 747 genes and harboring 12 secondary metabolite biosynthetic gene clusters.The surfactin biosynthetic gene cluster includes the core genes srfAA,srfAB,and srfAC.Surfactin produced by C5A-1 significantly enhanced the plant height,stem diameter,and aboveground and underground dry biomass of Populus tomentosa,Zea mays and Glycine max.[Conclusion]C5A-1 with high-producing surfactin was a novel strain belonging to B.velezensis,and the C14-and C15-surfactin A isoforms produced by C5A-1 demonstrated significant plant growth-promoting activity,which provides an optimized chassis cell factory for improving the surfactin yield by metabolic engineering strategies,and established a theoretical foundation for the practical application of surfactin in agricultural and forestry systems.关键词
贝莱斯芽胞杆菌/surfactin/MALDI-TOF MS/全基因组测序/次级代谢产物基因簇Key words
Bacillus velezensis/surfactin/MALDI-TOF MS/whole-genome sequencing/secondary metabolite biosynthetic gene clusters引用本文复制引用
吕镇,甘恬,霍思羽,赵晨笛,赵梦瑶,李亚涛,马玉超,耿玉清..产Surfactin贝莱斯芽胞杆菌C5A-1的鉴定和所产Surfactin对植物的促生效果[J].生物技术通报,2025,41(9):265-276,12.基金项目
国家重点研发项目(2021YFD220120302,2023YFD1501900),工业生物催化教育部重点实验室(清华大学)开放基金项目(2023001) （2021YFD220120302,2023YFD1501900）
