生物技术通报2025，Vol.41Issue(9)：277-288,12.DOI:10.13560/j.cnki.biotech.bull.1985.2025-0306
哈茨木霉M408的分离鉴定、生物学特性及对番茄早疫病的生防效果
Isolation,Identification,Biological Characteristics and Biocontrol Effects of Trichoderma harzianum M408 against Tomato Early Blight
摘要
Abstract
[Objective]Tomato early blight caused by Alternaria solani is a main disease of tomatoes,a biocontrol strain with good antagonistic effect against tomato early blight was isolated and identified from the rhizosphere soil of zucchini after tomato-zucchini rotation.[Method]Antagonistic strains were identified using morphological observation and molecular biology techniques.Its optimal biological indicators such as carbon source,temperature,light,and pH value were conducted.Its biocontrol effects were evaluated through indoor confrontation and field control experiments.[Result]A superior strain M408 was screened to antagonize tomato early blight,and was identified as Trichoderma harzianum through morphological observation and ITS sequence analysis,the optimal carbon source for both nutritional growth and spore production was glucose;the optimal growth temperature was 27℃;the mycelial growth rate was the fastest in total darkness,and the spore production was the highest under full light,and the optimal pH was 6.In the vitro plate confrontation culture test,the inhibition rate of strain 408 against Alternaria solani was 100%,and the antagonistic index of strain M408 against A.solani reached level I.The inhibition rates of M408 strain original spore powder agent with spore concentration of 108,107,106,and 105 CFU/mL against A.solani W5 were 100%,100%,98.51%,and 93.43%,respectively,which were all higher than the inhibition rate of 89.55%of the chemical fungicide 325 g/L difenoconazole azoxystrobin against A.solani W5.In the field efficacy test,7 d after the second spray,the biocontrol effect of T.harzianum M408 on early blight of tomato was obvious.The control effects of T.harzianum M408 strain original spore powder agent with spore concentration of 108,107,106 and 105 CFU/mL on tomato early blight were 89.44%,87.07%,84.56%and 81.01%,respectively,which were all higher than the relative control effect of 76.37%of 325 g/L difenoconazole azoxystrobin.[Conclusion]The strain of T.harzianum M408 is isolated from the rhizosphere soil of zucchini after tomato-zucchini rotation,it has a good biocontrol effect on early blight of tomato.It is a fungal resource with good biocontrol potential and has the value of further development and utilization.关键词
哈茨木霉M408/分离鉴定/生物学特性/番茄早疫病/拮抗作用Key words
Trichoderma harzianum M408/isolation and identification/biological characteristics/tomato early blight/antagonistic activity引用本文复制引用
苏秀敏,韩文清,王佼,李鹏,王秋兰,李万星,曹晋军..哈茨木霉M408的分离鉴定、生物学特性及对番茄早疫病的生防效果[J].生物技术通报,2025,41(9):277-288,12.基金项目
山西省基础研究计划青年科学研究项目(202303021222057,202203021212447),国家重点研发计划项目子课题(2021YFD1901101-5),山西农业大学谷子研究所科研项目(GZS202301) （202303021222057,202203021212447）
