生物技术通报2025，Vol.41Issue(9)：289-301,13.DOI:10.13560/j.cnki.biotech.bull.1985.2025-0215
根瘤菌Bd1的全基因组分析及TetR3对细胞生长和结瘤的负调控功能
Whole Genome Analysis of Bradyrhizobium sp.Bd1 and the Negative Regulating Function of TetR3 during Cell Growth and Nodulation
摘要
Abstract
[Objective]This research is aimed to understand the genetic background of Bradyrhizobium sp.Bd1 at the genome level,and explore effective ways to promote the growth and nodules of Bd1.[Method]Illumina+PacBio third-generation sequencing platform was used to sequence the whole genome of Bd1.Bioinformatics method was employed to analyze the species identification,functional gene annotation,nitrogen fixation-related genes and TetR family transcription factors.TetR3 gene knockout mutant was constructed by homologous recombination double-exchange method to investigate the function of TetR3 in the growth and nodulation of Bd1.[Result]The genomic average nucleotide consistency(ANI)and digital DNA-DNA hybridization values between Bd1 and Bradyrhizobium diazoefficiens USDA110 were 99.98%and 98.7%,respectively.Bd1 genome size was 9 009 469 bp with 64.1%guanine-cytosine content,8 403 coding sequences,within them 51 tRNA coding genes and 3 sets of ribosomal RNA genes.The number of nod,nif and fix genes were 9,12 and 10,respectively.There were 58 genes encoding TetR family transcription factors with diverse structure.Compared with the wild Bd1,the growth and nodulation capacity were significantly enhanced,and glutathione S-transferase activity increased by 41.0%in TetR3-inactive mutant(Bd1ΔTetR3).[Conclusion]Bd1 is a novel strain belonging to B.diazoefficiens,which has the ability of nitrogen fixation in symbiotic nodulation with soybean.TetR3 negatively regulates the growth and nodulation in Bd1 by balancing cell REDOX capacity.关键词
慢生根瘤菌/全基因组测序/TetR/大豆/谷胱甘肽S-转移酶Key words
Bradyrhizobium/whole genome sequencing/TetR/soybeans/glutathione S-transferase引用本文复制引用
李亚涛,张志鹏,赵梦瑶,吕镇,甘恬,魏浩,吴书凤,马玉超..根瘤菌Bd1的全基因组分析及TetR3对细胞生长和结瘤的负调控功能[J].生物技术通报,2025,41(9):289-301,13.基金项目
国家重点研发计划(2021YFD220120302,2023YFD1501900),工业生物催化教育部重点实验室(清华大学)开放基金项目(2023001) （2021YFD220120302,2023YFD1501900）
