生命科学研究2025，Vol.29Issue(4)：283-290,8.DOI:10.16605/j.cnki.1007-7847.2024.12.0208
Drr1的表达缺失损害小鼠的认知功能
Deficiency in Drr1 Expression Impairs Cognitive Function in Mice
摘要
Abstract
In order to explore the role and mechanism of Drr1 in aging and aging-related cognitive changes,Drr1 knockout mice were used to conduct behavioral experiments such as new object recognition,Y-maze,water maze and nesting to assess their cognitive abilities.Immunofluorescence staining was performed on mouse brain tissue sections to analyze the changes in the number of neurons in the brain.At the same time,transcriptome sequencing analysis was conducted on mouse brain tissue samples to identify the molecular pathways related to Drr1 expression and potential downstream factors.Finally,real-time quantitative PCR was used to verify the sequencing results.The results showed that,compared with wild-type mice,Drr1 knockout mice exhibited significant cognitive impairment,along with a significant reduction in neuronal numbers in specific brain regions.Moreover,there were changes in signaling pathways related to metabolism,oxidative stress,and cognition in the brain tissues of Drr1 knockout mice.These results suggest that the loss of Drr1 expression may lead to cognitive decline in mice by causing disorders of metabolism and promoting oxidative stress.关键词
肾细胞癌下调因子1(Drr1)/认知能力/代谢/氧化应激/衰老Key words
down-regulated in renal cell carcinoma 1(Drr1)/cognitive ability/metabolism/oxidative stress/aging分类
生物科学引用本文复制引用
袁子静,潘旭亿,林雪,卜婉婷,朱琳,卢方晋,牟萍..Drr1的表达缺失损害小鼠的认知功能[J].生命科学研究,2025,29(4):283-290,8.基金项目
教育部"春晖计划"合作科研项目(HZKY20220427) （HZKY20220427）
2023年辽宁省大学生创新创业训练计划项目(S202310164004) （S202310164004）
辽宁省教育厅自主选题项目(LJ212410164008) （LJ212410164008）
辽宁省科技计划联合计划技术攻关计划项目(2024JH2/102600232) （2024JH2/102600232）
