生命科学研究2025，Vol.29Issue(4)：304-311,8.DOI:10.16605/j.cnki.1007-7847.2024.12.0204
基于网络药理学-分子对接和动物实验探究达格列净对大鼠痛风性关节炎的预防与治疗作用
Preventive and Therapeutic Effects of Dapagliflozin on Gouty Arthritis in Rats Based on Network Pharmacology-Molecular Docking and Animal Experiments
摘要
Abstract
In order to explore the preventive and therapeutic effects of dapagliflozin(DAPA)on gouty arthri-tis(GA)in rats,the signaling pathways of DAPA in the treatment of GA were screened using network pharma-cology and molecular docking technology,and the GA model of SD rats was constructed by injecting sodium urate crystals into the ankle.The swelling degree of the ankle was measured by a vernier caliper,and the pathological changes of the ankle were observed by hematoxylin and eosin staining.The levels of inflamma-tory factors were detected by enzyme-linked immunosorbent assay,and the expression of nucleotide binding oligomerization domain(NOD)-like receptor family pyrin domain containing 3(NLRP3)and caspase-1 in the synovial tissues of the ankle joint was detected by Western-blot.The results of network pharmacology and molecular docking showed that the potential targets of DAPA in the treatment of GA were significantly en-riched in the NOD-like receptor signaling pathway(P＜0.01),and the binding energies of all core targets in this pathway to DAPA were lower than-29.29 kJ/mol.Animal experiments showed that DAPA significantly reduced ankle swelling,ameliorated pathological injury,decreased serum levels of inflammatory cytokines interleukin-1β(IL-1β)and tumor necrosis factor-α(TNF-α),and suppressed the expression of NLRP3 and caspase-1 in synovial tissues of GA rats.These results suggest that DAPA may alleviate acute inflammatory responses of GA rats by inhibiting the NLRP3/caspase-1 signaling pathway.关键词
痛风性关节炎(GA)/达格列净(DAPA)/网络药理学/NLRP3/caspase-1信号通路/大鼠Key words
gouty arthritis(GA)/dapagliflozin(DAPA)/network pharmacology/NLRP3/caspase-1 signaling pathway/rat分类
生物科学引用本文复制引用
叶涛,杜静芳,李偏,申娜..基于网络药理学-分子对接和动物实验探究达格列净对大鼠痛风性关节炎的预防与治疗作用[J].生命科学研究,2025,29(4):304-311,8.基金项目
河北省科技厅卫生健康创新专项(21377797D) （21377797D）
河北省医学科学研究课题计划(20250166) （20250166）
