生态环境学报2025，Vol.34Issue(9)：1452-1462,11.DOI:10.16258/j.cnki.1674-5906.2025.09.012
珠江广州河段粪便污染和溶解有机质的来源解析及分布特征
Source Analysis and Distribution Characteristics of Fecal Pollution and Dissolved Organic Matter in the Guangzhou Section of the Pearl River
摘要
Abstract
Accurately identifying the sources of fecal contamination and dissolved organic matter(DOM)pollution is critical for water quality management and maintaining ecosystem health.Fluorescence quantitative polymerase chain reaction(qPCR),three-dimensional fluorescence spectroscopy(3D-EEM),and parallel factor analysis(PARAFAC)were used to analyze the sources of fecal pollution and DOM,their distribution characteristics in the Guangzhou section of the Pearl River,and the potential relationship between them.The results showed that the average detection concentrations of the human-associated Bacteroides marker(BacHum)and crAssphage marker(CPQ_064)in the Guangzhou section of the Pearl River were(4.56±0.58)and(4.18±0.56)log10(gene copies/100 mL),respectively.No fecal markers from other sources were detected,indicating that fecal pollution in this section of the river originated from human feces.The 3D-EEM-PARAFAC analysis identified three primary DOM components:microbially-derived humic substances(C1),tryptophan-like substances(C2),and terrestrial derived humic substances(C3).In the dry and calm seasons,DOM was predominantly derived from endogenous inputs,whereas terrestrial inputs became the main source during the wet season.During the wet season,both fecal and DOM pollution were more severe.Along the flow direction of the Guangzhou section of the Pearl River,the levels of fecal matter and DOM pollution first increased and then decreased.Pearson's correlation analysis revealed strong and significant positive correlations between BacHum,CPQ_064,and C1,C2,and C3(p<0.001).There was also a positive correlation between total phosphorus(TP),ammonia nitrogen(NH3-N),BacHum,and CPQ_064(p<0.05),indicating that they exhibited pollution homology.The positive correlations between fecal markers and DOM components suggest that 3D-EEM analysis can be used as a cost-effective and timely pre-detection method for detecting fecal pollution in water.This study provides new insights into the sources,distribution,and relationships between fecal pollution and DOM in the Guangzhou section of the Pearl River and serves as a strong technical guide for water pollution control and management in this region.关键词
粪便污染/溶解性有机质/污染源解析/分布特征/珠江广州河段Key words
fecal pollution/dissolved organic matter/pollution source analysis/distribution characteristics/Guangzhou section of Pearl River分类
资源环境引用本文复制引用
蒋凯,柯常栋,王丽萍,李朋辉,赖迪智,张杨,吴永洁,吴仁人,肖利平..珠江广州河段粪便污染和溶解有机质的来源解析及分布特征[J].生态环境学报,2025,34(9):1452-1462,11.基金项目
国家自然科学基金项目(42407366) （42407366）
中国博士后科学基金资助项目(2024M752163) （2024M752163）
中央级公益性科研院所基本科研业务专项资助(PM-zx703-202406-165 （）
PM-zx703-202406-236) （）
广州市科技计划项目(202201011440) （202201011440）
