西安交通大学学报（医学版）2025，Vol.46Issue(5)：818-823,6.DOI:10.7652/jdyxb202505015
和厚朴酚通过Raf/MEK/ERK通路调控糖酵解抑制胰腺癌细胞恶性进展
Honokiol inhibits the malignant progression of pancreatic cancer cells by regulating glycolysis through the Raf/MEK/ERK pathway
摘要
Abstract
Objective To explore the effects of honokiol on malignant phenotypes in PANC-1 cells and its mechanisms.Methods In our experiments,MTT assay was used to detect the effects of honokiol on PANC-1 cell viability.The migration and invasion of PANC-1 cells were evaluated by wound healing assay and Transwell assay.The glycolysis level was detected by ELISA and ECAR.Western blotting was used to detect the expressions of OCT4,SOX2,Nanog,LDHA,HK2 and Raf/MEK/ERK pathway related proteins.Results Honokiol could significantly inhibit the migration and invasion abilities of PANC-1 cells(migration rate and invasion rate being 13.78％-59.45％and 12.47％-44.47％,respectively).Mechanistically,honokiol targeted Raf/MEK/ERK signaling pathway and significantly reduced the expressions of LDHA(29.60％)and HK2(31.98％),thereby reducing glycolysis and lactate production(29.42％-56.31％,compared with control group P＜0.05).This metabolic reprogramming effect ultimately inhibited the protein expressions of OCT4(33.38％),SOX2(33.07％),and Nanog(26.24％).Conclusion Honokiol treatment can inhibit the cell viability,migration and invasion abilities of PANC-1 cells.The mechanisms are related to the inhibition of glycolysis and cell stemness by regulating Raf/MEK/ERK pathway.关键词
和厚朴酚/胰腺癌/细胞干性/糖酵解/ERKKey words
honokiol/pancreatic cancer/cell stemness/glycolysis/ERK分类
医药卫生引用本文复制引用
刘鑫,李晶,王玺,董蕾..和厚朴酚通过Raf/MEK/ERK通路调控糖酵解抑制胰腺癌细胞恶性进展[J].西安交通大学学报（医学版）,2025,46(5):818-823,6.基金项目
国家自然科学基金青年基金资助项目(No.82100657) （No.82100657）
陕西省自然科学基础研究计划项目(No.2020JQ-935)Supported by the National Natural Science Foundation of China-Youth Science Fund Project(No.82100657)and Natural Science Basis Research Plan of Shaanxi Province of China(No.2020JQ-935) （No.2020JQ-935）
