西安交通大学学报（医学版）2025，Vol.46Issue(5)：852-859,8.DOI:10.7652/jdyxb202505019
SCRIB通过激活JAK-STAT3信号通路对胶质母细胞瘤增殖、凋亡和自噬的影响
Effects of SCRIB on proliferation,apoptosis and autophagy of glioblastoma cells by activating JAK-STAT3 signaling pathway
摘要
Abstract
Objective To investigate the effects of scribble planar cell polarity protein(SCRIB)on proliferation,apoptosis,and autophagy of glioblastoma(GBM)and elucidate its potential underlying mechanisms.Methods The expression level of SCRIB in GBM tissue was queried through the Biomarker Exploration of Solid Tumors(BEST)database.Lentivirus-mediated shRNA interference was employed to downregulate SCRIB expression in human glioblastoma cell lines U87 and U251,which were divided into negative control group(mock)and SCRIB shRNA interference groups(kd1 and kd2).SCRIB expression levels were detected using Western blotting(WB)and quantitative polymerase chain reaction(qPCR).EdU incorporation and cell apoptosis rates were detected by flow cytometry(FCM).CCK-8 assay was used to detect the proliferation vitality of U87 and U251 cells,and WB was used to detect the expression of proliferation-related proteins.Immunofluorescence(IF)staining was conducted to detect the expression of autophagy-related proteins LC3 and p62,followed by quantitative analysis across multiple fields.WB was also used to detect the expression levels of LC3,p62,and proteins in the JAK-STAT3 signaling pathway.Results Compared with that of normal tissues,SCRIB mRNA expression level was significantly upregulated in GBM tissues(P＜0.05).FCM results showed that EdU incorporation rates were significantly reduced(P＜0.001)while cell apoptosis rates were markedly increased(P＜0.001)in U87 and U251 cells with SCRIB knockdown.CCK-8 results indicated that compared with the mock group,the proliferation vitality of U87 and U251 cells in the SCRIB knockdown group was significantly downregulated(P＜0.001).IF staining showed that LC3 fluorescence aggregation was significantly enhanced(P＜0.001),while p62 fluorescence aggregation was significantly reduced(P＜0.001)in the SCRIB knockdown group.WB results showed that compared with the mock group,the protein expression levels of p27,LC3,p-JAK2 and p-STAT3 were upregulated,while C-Myc,Cyclin D1,MCM,PCNA and p62 were downregulated,with statistically significant differences(P＜0.05).Conclusion Downregulation of SCRIB may induce autophagy and apoptosis in glioblastoma cells by inhibiting the JAK-STAT3 signaling pathway,thereby suppressing cell proliferation.关键词
胶质母细胞瘤(GBM)/SCRIB/增殖/凋亡/自噬/JAK-STAT3Key words
glioblastoma(GBM)/scribble planar cell polarity protein(SCRIB)/proliferation/apoptosis/autophagy/JAK-STAT3分类
医药卫生引用本文复制引用
姚晓含,王志清,姚明辰,李丹阳,李赫阳,申心怡,张骞,韩滨..SCRIB通过激活JAK-STAT3信号通路对胶质母细胞瘤增殖、凋亡和自噬的影响[J].西安交通大学学报（医学版）,2025,46(5):852-859,8.基金项目
河南省创新研发专项项目(No.HNGD2024005)Supported by Henan Province Innovation and Research and Development Special Project(No.HNGD2024005) （No.HNGD2024005）
