畜牧兽医杂志2025，Vol.44Issue(5)：71-76,6.DOI:10.7606/j.issn.1004-6704.2025.05.012
羊感染布鲁氏菌血清非靶向代谢组学研究
Non-targeted Metabolomics of Serum Infections with Brucella Abortus in Sheep
摘要
Abstract
To investigate the alterations of small-molecule metabolites in the serum of brucella-infected sheep in Qinghai province,this study employed ultra-high-performance liquid chromatography coupled with quadrupole-orbitrap high-resolution mass spectrometry(UHPLC-QExactive-Orbitrap MS/MS)to analyze the metabolomic profiles of sheep serum infected with Brucella.The results revealed 15 significantly differential metabolites between three infected sheep and three healthy sheep.A-mong these,except for isotretinoin,kynurenic acid,phenylacetylglycine,eicosapentaenoic acid,and(±)12(13)-DiHOME,the levels of the remaining 10 differential metabolites were decreased.KEGG pathway analysis indicated that these differential metab-olites were primarily enriched in key metabolic pathways such as phenylalanine,tyrosine,and tryptophan biosynthesis;histidine metabolism;the pentose phosphate pathway;and primary bile acid biosynthesis.This study elucidates the characteris-tics of host metabolic dysregulation induced by brucella in-fection from a metabolomic perspective.The identified differ-ential metabolites provide potential molecular markers for the early diagnosis of brucellosis,while also laying an impor-tant theoretical foundation for a deeper understanding of its pathogenic mechanisms and the development of novel pre-vention and control strategies.These findings hold significant scientific value for the healthy development of animal hus-bandry and public health security.关键词
布鲁氏菌病/羊/血清/非靶向代谢组学Key words
brucellosis/sheep/serum/untargeted metabolo-mics分类
农业科技引用本文复制引用
牛生莲,李强,王建玲,马丽,李积权,赵延梅,薛红梅,赵志军..羊感染布鲁氏菌血清非靶向代谢组学研究[J].畜牧兽医杂志,2025,44(5):71-76,6.基金项目
2023年度青海省"昆仑英才·科技领军人才"自然科学与工程技术学科带头人培养计划 （）
2024年度青海省"昆仑英才·高层次卫生健康人才"培养省级卫生健康示范团队项目 （）
