西南林业大学学报2025，Vol.45Issue(7)：40-48,9.DOI:10.11929/j.swfu.202407005
添加不同培养基质对牛樟芝生长特性及总三萜积累的影响
Effects of Different Cultivation Substrates on Growth Characteristics and Total Triterpenoid Accumulation of Taiwanofungus camphoratus
摘要
Abstract
In this study,the basic potato dextrose agar(PDA)medium with different concentrations of Cin-namomum cassia and Phoebe zhennan tender branches,tender leaves,and a mixture of branches and leaves,was used for Taiwanofungus camphoratus culture medium to explore its effect on the growth characteristics of T.cam-phoratus mycelium and total triterpenoid accumulation.The results showed that adding C.cassia and P.zhennan substrates in PDA medium significantly affected the growth,biomass and total triterpene accumulation of T.cam-phoratus.Regarding the effect of substrate concentration,adding 16-32 g/L of C.cassia tender branches signific-antly promoted the mycelium growth and total triterpene accumulation of T.camphoratus,and the total triterpene content was significantly increased by 142.67-207.85％compared with the control.Additionally,4 g/L C.cassia branches and leaves mixture and 8 g/L P.zhennan tender leaves substrates significantly increased the fresh weight and dry weight of T.camphoratus by 196.00％and 112.50％,respectively.Comparing the type of added substrate organs,it was found that compared with tender leaves and branches and leaves mixture substrate,the addition of C.cassia and P.zhennan tender branches effectively promoted the growth of T.camphoratus mycelium.Further-more,C.cassia tender branches and P.zhennan branches and leaves mixture effectively improved the total triter-penoid content of T.camphoratus.When comparing the substrates added,it was observed that adding C.cassia substrate was more effective in promoting the growth characteristics,fresh weight and total triterpene content of T.camphoratus,while the P.zhennan substrate was more favorable for the dry weight accumulation of T.cam-phoratus.In conclusion,the results indicated that adding C.cassia tender branches at a concentration of 16-32 g/L to the PDA medium had the optimal effect on promoting the growth characteristics of T.camphoratus and had the most significant effect on enhancing total triterpenoid accumulation.关键词
牛樟芝/肉桂/楠木/总三萜/生长特性Key words
Taiwanofungus camphoratus/Cinnamomum cassia/Phoebe zhennan/total triterpene/growth characteristic分类
农业科技引用本文复制引用
张红玲,张静,武自强,张颖,郑元..添加不同培养基质对牛樟芝生长特性及总三萜积累的影响[J].西南林业大学学报,2025,45(7):40-48,9.基金项目
国家自然科学基金项目(32160736)资助 （32160736）
云南省农业基础研究联合专项重点项目(202101BD070001-020)资助 （202101BD070001-020）
云南省基础研究计划面上项目(202101AT070044)资助 （202101AT070044）
"兴滇英才支持计划"青年人才专项项目(XDYC-QNRC-2022-0245)资助. （XDYC-QNRC-2022-0245）
