西南林业大学学报2025，Vol.45Issue(9)：55-62,8.DOI:10.11929/j.swfu.202409007
法师系多肉植物人工杂交授粉的影响因素研究
Study on the Key Technology of Artificial Cross-pollination of Aeonium Series Succulents
摘要
Abstract
In order to screen the key factors affecting the cross-pollination of Aeonium series succulents and optimize the artificial cross-pollination technology,the pollen and stigma activity of 7 Aeonium series succulent varieties were detected by TTC staining,in vitro germination and benzidine-hydrogen peroxide method to screen the appropriate pollination time and cross parent.'Voodoo'(♀)×'Green Velour'(♂)as a cross-pollination com-bination,the effects of different pollination methods,gibberellin concentration,wet bag interval on the changes of pistil oxidase activity and malondialdehyde content were compared to explore the key techniques for artificial cross-pollination of Aeonium series succulent plants.The results showed that the pollen and stigma activity was strongest in most varieties at the early flowering stage,which is a suitable period for pollination.Pollen and stigma activity varied significantly among varieties,with'Voodoo','Green Velour'and'Short Black'having high-er pollen vigor and'Black Kingkong'and'Ink Painting'were lower.The stigmas were more fertile for'Voodoo','Short Black'and'Black Kingkong',and'Green Velour','Halloween'were less fertile.Gibberellin concentration,wet bag interval and pollination method had significant effects on pistil oxidase activity and malondialdehyde con-tent after hybridization,30 mg/LGA,2 h wet bag interval and three treatments of repeated pollination were the most effective,and POD,SOD,β-1,3-glucanase activities were at high levels in pistils and MDA content was lower in 4-24 h after cross-pollination.关键词
杂交授粉/花粉活力/柱头活性/保护酶活性/影响因素/3-葡聚糖酶活性Key words
cross-pollination/pollen viability/stigma activity/protectase activity/influencing factor/3-glu-canase activity分类
农业科技引用本文复制引用
李素华,孟彤,倪文婷,韩浩章,赵荣,张丽华,王芳,张楠..法师系多肉植物人工杂交授粉的影响因素研究[J].西南林业大学学报,2025,45(9):55-62,8.基金项目
江苏省高等学校自然科学基金项目(23KJD210005)资助 （23KJD210005）
苏北科技专项(SZ-SQ2021011)资助 （SZ-SQ2021011）
宿迁市科技计划项目(K202207)资助. （K202207）
