西南林业大学学报2025，Vol.45Issue(9)：70-78,9.DOI:10.11929/j.swfu.202310015
民族药小白及与近缘种叶绿体基因组密码子偏好性分析
Analysis of Codon Usage Bias in the Chloroplast Genome of Bletilla and its Related Species
摘要
Abstract
To acquire a more comprehensive understanding of the genetic traits of of Bletilla plants,an ana-lysis was conducted on the codon usage patterns and primary influencing factors of 4 species belonging to the genus.The neutrality plot,ENC-plot,and PR2-plot analysis of chloroplast genome codons in 4 Bletilla plants of B.striata,B.formosana,B.sinensis,B.ochracea were conducted based on CUSP,Codon W 1.4.2 and EMBOSS software.The study revealed that 4 optimal codons shared by these four species all exhibit the characteristic that the third base is A/U,and the effective number of codons(ENC)of all protein-coding genes exceeds the threshold of 35.This phenomenon reflects a relatively weak trend in codon preference in Bletilla plants.Furthermore,thr-ough parity preference(PR2-Plot)analysis,the study observed that the distribution of genes in 4 regions is not bal-anced;and effective codon number analysis(ENC-Plot)showed that most genes are located below the standard curve.Furthermore,in the neutral plot analysis,all genes were significantly shifted upward from the diagonal.This series of analyzes collectively shows that codon usage bias is affected by both mutation and natural selection,but natural selection plays a more critical role in this process and has a more significant impact on codon preference.关键词
白及/小白及/华白及/黄花白及/叶绿体基因组/密码子偏好性/最优密码子Key words
Bletilla striata/Bletilla formosana/Bletilla sinensis/Bletilla ochracea/chloroplast genome/codon usage bias/optimal codon分类
生物科学引用本文复制引用
张玥,赵飞亚,蒋艳,陶爱恩..民族药小白及与近缘种叶绿体基因组密码子偏好性分析[J].西南林业大学学报,2025,45(9):70-78,9.基金项目
丽江市科技计划项目(2022LJSHFZ010)资助 （2022LJSHFZ010）
校级中青年学术和技术带头人后备人才项目资助 （）
丽江市中青年学术和技术带头人后备人才项目资助. （）
