西北林学院学报2025，Vol.40Issue(5)：122-130,9.DOI:10.3969/j.issn.1001-7461.2025.05.12
马尾松磷代谢相关基因PmGDPD1克隆与表达分析
Cloning and Expression Analysis of Phosphorus Metabolism-related Gene PmGDPD1 in Pinus massoniana
摘要
Abstract
GDPD1 gene is critical for the regulation of phospholipid metabolism in plants,which plays an important role in stress resistance of plants.PmGDPD1 gene was cloned and its expression under low phosphorus stress was examined,in order to provide a basis for the study of PmGDPD1 function.PmGD-PD1 gene was cloned by RT-PCR and bioinformatics analysis was performed through an online website.Subcellular localization was analyzed by the transient transformation system in tobacco.Expression of this gene in plant tissues under low phosphorus stress was determined by RT-qPCR.The longest coding region of PmGDPD1 gene is 1 083 bp in length,encoding 361 amino acids and containing a conserved GDPD do-main.PmGDPD1 was clustered with its homologs in tobacco,wheat,and rice.Subcellular localization ex-periments showed that the protein was mainly localized in the cell membrane and expressed in small amounts in the nucleus.RT-qPCR showed that the expression of PmGDPD1 gene was induced after 24,48 and 60 days of low phosphorus stress in P.massoniana seedlings,and the expression was induced in differ-ent tissues.PmGDPD1 gene,which mainly functions at cell membranes,responds to low phosphorus stress,providing a reference for further exploring the mechanism of low phosphorus stress response of P.massoniana.关键词
马尾松/低磷胁迫/PmGDPD1/亚细胞定位Key words
Pinus massoniana/low phosphorus stress/PmGDPD1/subcellular localization分类
农业科技引用本文复制引用
王君荣,席梓晴,范付华..马尾松磷代谢相关基因PmGDPD1克隆与表达分析[J].西北林学院学报,2025,40(5):122-130,9.基金项目
国家自然科学基金项目(32460383). （32460383）
