现代中西医结合杂志2025，Vol.34Issue(14)：1911-1917,1992,8.DOI:10.3969/j.issn.1008-8849.2025.14.003
大豆苷元对肥胖型妊娠期糖尿病孕鼠肝脏脂质代谢的影响研究
Effects of daidzein on liver lipid metabolism in obese pregnant rats with gestational diabetes
摘要
Abstract
Objective It is to investigate the effects of daidzein on liver lipid metabolism in obese pregnant rats with gestational diabetes and its potential mechanisms.Methods Sixty female C57BL/6J mice were taken,in which 10 mice were randomly selected as normal group,and the remaining mice were fed with high-fat/high-sugar diet and given streptozo-tocin by intraperitoneal injection after mating to establish models of obese gestational diabetes.The successfully modeled mice were randomly divided into 5 groups,the metformin group was given metformin 50 mg/(kg·d)by gavage,the low-,medium-,and high-dose groups of daidzein were given daidzein 25 mg/(kg·d),50 mg/(kg·d),and 100 mg/(kg·d)by gavage,respectively,the model group and normal group were given equivalent volumes of physiological saline by ga-vage,all administered once daily.After 2 weeks of continuous gavage,the pregnant mice were weighed,and the levels of fasting blood glucose,insulin,insulin resistance index,and liver function indicators[aspartate aminotransferase(AST),alanine aminotransferase(ALT)],lipid profiles[total cholesterol(TC),triglycerides(TG),high-density lipoprotein cholesterol(HDL-C),low-density lipoprotein cholesterol(LDL-C)],inflammatory factors[interleukin-1α(IL-1α),tumor necrosis factor-α(TNF-α)],oxidative stress markers[superoxide dismutase(SOD),glutathione(GSH)]were measured,the histopathological morphology and ultrastructure of liver tissue were observed via HE staining and transmission electron microscopy,and the expressions of lipid metabolism-related proteins in liver tissue[sterol regulatory element-bind-ing protein-1c(SREBP-1c),fatty acid synthase(FAS),peroxisome proliferator-activated receptor alpha(PPARα),carnitine palmitoyl transferase-1(CPT-1)and acyl-CoA oxidase 1(ACOX1)]were detected by Western blot method.Re-sults Compared with the normal group,the body weight,fasting blood glucose,insulin resistance index,and serum levels of AST,ALT,TG,TC,LDL-C,IL-1α and TNF-α,as well as relative protein expressions of SREBP-1c and FAS in liver tissue were all significantly increased in the pregnant mice of model group(all P<0.05),while the serum levels of insulin,HDL-C,SOD and GSH,as well as the relative protein expressions of PPARα,CPT-1 and ACOX1 in liver tissue were all significantly decreased(all P<0.05),fatty vacuoles and mitochondrial damage were observed in liver tissue.Compared with the model group,the body weight,fasting blood glucose and insulin resistance index were all significantly decreased in pregnant mice of the metformin group(all P<0.05),no significant changes in these parameters were observed in each dose group of daidzein(all P>0.05);the serum levels of AST,ALT,TG,TC,LDL-C,IL-1α and TNF-α,as well as relative protein expressions of SREBP-1c and FAS in liver tissue were all significantly decreased in the pregnant mice of metformin group and each dose groups of daidzein(all P<0.05),while the serum levels of insulin,HDL-C,SOD and GSH,as well as the relative protein expressions of PPARα,CPT-1 and ACOX1 in liver tissue were all significantly in-creased(all P<0.05);the hepatic steatosis and mitochondrial damage were obviously alleviated in all medicated groups.Conclusion Daidzein can improve lipid metabolism,inhibit inflammation and oxidative stress,and alleviate hepatic steato-sis and mitochondrial damage in obese pregnant rats with gestational diabetes.The mechanism may be related to regulate fatty acid synthesis and degradation.关键词
大豆苷元/肥胖型妊娠期糖尿病/胰岛素抵抗/脂肪酸代谢Key words
daidzein/obese gestational diabetes/insulin resistance/fatty acid metabolism分类
医药卫生引用本文复制引用
华蕾,徐小琼,李春健,邵文璠,李顺序..大豆苷元对肥胖型妊娠期糖尿病孕鼠肝脏脂质代谢的影响研究[J].现代中西医结合杂志,2025,34(14):1911-1917,1992,8.基金项目
云南省科技计划重点项目(202101BA070001-223) （202101BA070001-223）
