针刺研究2025，Vol.50Issue(9)：1013-1020,8.DOI:10.13702/j.1000-0607.20240547
电针通过脂肪组织SIRT1调节Th17/Treg细胞平衡改善胰岛素抵抗肥胖的机制研究
Mechanism of electroacupuncture in improving obesity with insulin resistance by regulating the balance of Th17 and Treg cells through SIRT1
摘要
Abstract
Objective To observe the effect of electroacupuncture(EA)on silent information regulator 1(SIRT1)and the balance of Th17 and Treg in the adipose tissues of obese rats with insulin resistance,and to explore the mechanisms by which EA improves insulin resistance and obesity.Methods Male Wistar rats were randomly divided into 5 groups:normal group,model group,EA group,sham EA group,and combination group,with 8 rats in each group.An insulin resistance obesity model was established using a high-fat diet.Rats in the EA group received EA at"Zhongwan"(CV12),"Guanyuan"(CV4),"Zusanli"(ST36),and"Fenglong"(ST40),with the needles retained for 10 min each time.The sham EA group underwent superficial needling 5 mm beside the acupoints of the EA group,with electrodes clamped but not powered,while the remaining procedures were identical to those in the EA group.The combination group was administered Sirtinol solution via tail vein injection,with the EA intervention identical to that in the EA group.All interventions were conducted 3 times a week(Monday,Wednesday,and Friday)for 8 weeks.Body mass,Lee's index and glucose infusion rate(GIR)were measured before and after the intervention.Intraperitoneal glucose tolerance tests(IPGTT)were conducted in the 6th week of the intervention period to test the blood glucose level.After the intervention,the percentages of Th17 and Treg cells in the adipose tissue were detected by flow cytometry and the Th17/Treg ratio was calculated,while the relative expression levels of SIRT1,acetylated nuclear factor-κB(AC-NF-κB),and interleukin-17 receptor A(IL-17A)proteins in the adipose tissue of rats in each group were analyzed by Western blot.Results Compared with the normal group,the model group showed increased body mass and Lee's index(P<0.05),elevated blood glucose level in IPGTT at all time pionts(P<0.05),decreased GIR(P<0.05),increased percentages of Th17 cells(P<0.05)and decreased percentages of Treg cells(P<0.05)in adipose tissue,and increased Th17/Treg ratio(P<0.05),the expression of SIRT1 protein was decreased(P<0.05)while the expression of Ac-NF-κB and IL-17A proteins were increased(P<0.05)in adipose tissue.Compared with the model group,the EA group showed a significant decrease in body mass and Lee's index(P<0.05),significantly reduced blood glucose level in IPGTT at all time points(P<0.05),increased GIR(P<0.05),decreased percentages of Th17 cells(P<0.05)and increased percentages of Treg cells(P<0.05)in adipose tissue,and reduced Th17/Treg ratio(P<0.05),the expression of SIRT1 protein was increased(P<0.05)while the protein expressions of Ac-NF-κB and IL-17A were decreased(P<0.05)in adipose tissue.Compared with the EA group,the combination group exhibited increases in body mass and Lee's index(P<0.05),elevated blood glucose level at 30,60,and 120 min post-glucose injection in IPGTT(P<0.05),and decreased GIR(P<0.05),the percentage of Th17 cells was increased(P<0.05)and the Th17/Treg ratio was larger(P<0.05)in adipose tissue,and the expression of SIRT1 protein was reduced(P<0.05)while the protein expressions of Ac-NF-κB and IL-17A were increased(P<0.05)in adipose tissue.Conclusion EA can reduce body mass and improve insulin sensitivity in obese rats,and its mechanism may be related to the activation of SIRT1,which regulates the balance of Th17/Treg and inhibits inflammatory responses.关键词
电针/肥胖/胰岛素抵抗/辅助性T细胞17/调节性T细胞/Th17/Treg平衡Key words
Electroacupuncture/Obesity/Insulin resistance/T helper cell 17/Regulatory T cell/Balance of Th17 and Treg引用本文复制引用
王文炎,刘静,黄裳,李勇,阮崇洁,贺涛..电针通过脂肪组织SIRT1调节Th17/Treg细胞平衡改善胰岛素抵抗肥胖的机制研究[J].针刺研究,2025,50(9):1013-1020,8.基金项目
湖北省中医药管理局中医药科研项目(No.ZY2023Q017) （No.ZY2023Q017）
武汉市卫生健康委"晨星"计划人才项目(No.武卫发[2023]28号) （No.武卫发[2023]28号）
