针刺研究2025，Vol.50Issue(9)：1021-1028,8.DOI:10.13702/j.1000-0607.20240585
针刺对佐剂性关节炎小鼠Mer受体酪氨酸激酶相关胞葬途径的影响
Effects of acupuncture on the efferocytosis pathway of Mer receptor tyrosine kinase in mice with adjuvant arthritis
摘要
Abstract
Objective To observe the effect of acupuncture at"Zusanli"(ST36)on the expression of Mer receptor tyrosine kinase(MerTK)-related efferocytosis regulatory molecules in mice with adjuvant arthritis induced by complete Freund's adjuvant(CFA),so as to explore the potential mechanism of acupuncture on efferocytosis in adjuvant arthritis.Methods C57BL/6J mice were randomly divided into a saline group,a model group,and an acupuncture group(10 mice/group).The right hind footpad of mice in the saline group was injected with 0.9%sodium chloride solution,while those in the model group and the acupuncture group were injected with CFA(50 μL)to establish the model of adjuvant arthritis.Mice in the acupuncture group received acupuncture at bilateral ST36 once a day for a total of 7 times,and mice in the saline group and the model group were fixed in the same way.During the experiment,paw swelling degree and the paw withdrawal thermal latency(PWTL)of mice were measured before modeling,on the day of modeling(D0)and on the 1st,3rd,5th and 7th day of acupuncture(D1,D3,D5,D7)to evaluate paw swelling and pain conditions.The mRNA expressions of tumor necrosis factor(TNF)-α,interleukin(IL)-6,and IL-1β in spinal cord tissue were detected by real-time quantitative PCR.The protein expressions of MerTK,phosphorylated(p)-signal transducer and activator of transcription 3(STAT3),STAT3,and programmed death-ligand 1(PD-L1)in footpad and spinal cord tissue were detected by Western blot.Results After modeling,compared with the saline group,the PWTL on D0—D7,the protein expression of PD-L1 in spinal cord and protein expressions of MerTK and PD-L1 in footpad,the phosphorylation level of STAT3 in spinal cord and footpad were decreased(P<0.01)in the model group,while the degree of paw swelling,mRNA expressions of TNF-α,IL-6,and IL-1β in spinal cord tissue were increased(P<0.01,P<0.05).Compared with the model group,the PWTL on D1—D7,the protein expressions of MerTK,PD-L1 and phosphorylation level of STAT3 in spinal cord and footpad were increased(P<0.01)in the acupuncture group after acupuncture intervention,while the degree of paw swelling on D7,the mRNA expressions of TNF-α,IL-6,and IL-1β in spinal cord tissue were significantly decreased(P<0.01,P<0.05).Conclusion Acupuncture can improve the symptoms of mice with adjuvant arthritis,which may be related to promoting MerTK-mediated efferocytosis and thus exerting an anti-inflammatory effect.关键词
针刺/佐剂性关节炎/胞葬/Mer受体酪氨酸激酶Key words
Acupuncture/Adjuvant arthritis/Efferocytosis/Mer receptor tyrosine kinase引用本文复制引用
赵季宇,苑功名,李佩云,王新梦,刘子瑄,徐媛,郭永明,李媛..针刺对佐剂性关节炎小鼠Mer受体酪氨酸激酶相关胞葬途径的影响[J].针刺研究,2025,50(9):1021-1028,8.基金项目
江苏省高等学校基础科学(自然科学)研究面上资助项目(No.22KJB360009) （自然科学）
江苏省科技计划项目基础研究计划自然科学基金青年项目(No.BK20230453) （No.BK20230453）
江苏省卫健委医学科研项目(No.Z2023032) （No.Z2023032）
国家自然科学基金青年项目(No.82004473) （No.82004473）
