针刺研究2025，Vol.50Issue(9)：1029-1036,8.DOI:10.13702/j.1000-0607.20240487
艾灸对类风湿关节炎模型大鼠滑膜组织cGAS/STING信号通路的影响
Effect of moxibustion on the cGAS/STING signaling pathway in synovial tissue of rats with rheumatoid arthritis
摘要
Abstract
Objective To investigate the effect of moxibustion on the cyclic GMP-AMP synthase(cGAS)/stimulator of interferon genes(STING)signaling pathway in the synovial tissue of knee joints in rheumatoid arthritis(RA)model rats,and to explore the mechanism by which moxibustion alleviates synovial inflammatory injury.Methods Forty male SD rats were randomly divided into 4 groups(10 rats in each group):blank,model,moxibustion,and triptolide groups.The RA model was induced by injecting complete Freund's adjuvant into the right hind toe combined with exposure to wind,cold,and damp environments.The moxibustion group received daily 20 min treatment at"Zusanli"(ST36)or"Shenshu"(BL23)for 15 d;the triptolide group was administered triptolide suspension(8 mg/kg)via gavage for 15 d.The joint swelling degree of right hind toe was measured by water displacement;the synovial morphology was assessed via HE staining;the mitochondrial ultrastructure was observed by transmission electron microscopy;the synovial protein expression levels of cGAS,STING,TANK-binding kinase 1(TBK1),phosphorylated interferon regulatory factor 3(p-IRF3)and phosphorylated nuclear factor-kappa B p65(p-NF-κB p65)were detected by Western blot;serum contents of interleukin-6(IL-6),intercellular adhesion molecule-1(ICAM-1),C-X-C motif chemokine ligand 10(CXCL10),and interferon-β(IFN-β)were measured by ELISA.Results Compared with the blank group,the model group showed increased joint swelling degree(P<0.01),irregular synovial lining cell distribution,marked hyperplasia,and inflammatory cell infiltration;synovial cGAS,STING,TBK1,p-IRF3,and p-NF-κB p65 protein expression levels and serum IL-6,ICAM-1,CXCL10,and IFN-β contents were significantly elevated(P<0.01).Compared with the model group,both moxibustion and triptolide interventions reduced joint swelling degree(P<0.01),improved synovial histopathology,and down regulated the aforementioned proteins and cytokines(P<0.01).No significant differences were observed between the moxibustion and triptolide groups.Conclusion Moxibustion attenuates joint swelling,inflammatory cytokine release,and synovial injury in RA model rats,potentially via suppression of the cGAS/STING pathway.关键词
艾灸/类风湿关节炎/环鸟苷酸-腺苷酸合成酶(cGAS)/干扰素基因刺激因子(STING)信号通路/炎性反应Key words
Moxibustion/Rheumatoid arthritis/cGAS/STING signaling pathway/Inflammatory response引用本文复制引用
彭传玉,蔡荣林,郝锋,刘磊,张传英,何璐,靳明杨..艾灸对类风湿关节炎模型大鼠滑膜组织cGAS/STING信号通路的影响[J].针刺研究,2025,50(9):1029-1036,8.基金项目
国家自然科学基金项目(No.82205289、82274645) （No.82205289、82274645）
安徽省自然科学基金青年项目(No.2208085QH265、2108085QH363) （No.2208085QH265、2108085QH363）
安徽高校自然科学研究重点项目(No.KJ2021A0571、2022AH050526) （No.KJ2021A0571、2022AH050526）
国家级大学生创新创业训练计划项目(No.202410369015) （No.202410369015）
