针刺研究2025，Vol.50Issue(9)：1064-1071,8.DOI:10.13702/j.1000-0607.20240573
电针少阳经特定穴调控P2Y12受体防治大鼠偏头痛慢性化的机制研究
Electroacupuncture of specific acupoints of Shaoyang Meridian relieves progression of chronic migrain by down-regulating expression of P2Y12 receptor in rats
摘要
Abstract
Objective To observe the effect of electroacupuncture(EA)at specific points of the Shaoyang Meridian on mechanical pain threshold,and expressions of P2Y12 receptor(P2RY12),ionic calcium binding molecule 1(Iba-1),calcitonin gene-related peptide(CGRP)and c-fos in rats with chronic migraine(CM),so as to explore its mechanisms underlying prevention and treatment of CM.Methods Forty-eight SD rats(half male and half female)were randomly divided into 6 groups(using a random number table method):control,model,EA,antagonist,EA+agonist,and medication groups,with 8 rats in each group.The CM model was established by repeated intraperitoneal injection of nitroglycerin(10 mg/kg,once every other day,5 times altogether).EA(10 Hz/50 Hz,a strength being able to induce local slight regular muscular contraction of the fore-limb)was applied to bilateral"Fengchi"(GB20),"Yanglingquan"(GB34),and"Waiguan"(TE5)for 30 min,once a day before modeling and once every other day during modeling,for a total of 6 times.The rats of the antagonist group received intrathecal injection of P2RY12 antagonist MRS2395(200 μg/rat),once every other day for a total of 5 times.Rats of the EA+agonist group received intrathecal injection of P2RY12 agonist ADP(4.272 μg/rat)once every other day for a total of 5 times,and those of the medication group received intraperitoneal injection of topiramate(30 mg/kg)once a day for 9 d.Rats of the other groups were bound for 30 min during EA.On the 1st,3rd,5th,7th,and 9th day of modeling,2 h before the intervention,the mechanical pain thresholds of the periorbital region of the eye and the plantar region of the hindlimb in each group were measured using a von Frey filament.The number of P2RY12 positive cells and the expression of P2RY12 mRNA in the caudal nucleus of the trigeminal spinal tract(TNC)were detected using immunofluorescence staining and real-time fluorescence quantitative PCR,separately,and the expression levels of P2RY12,Iba-1,CGRP,and c-fos proteins in the TNC tissue were detected using Western blot.Results In comparison with the control group,the periorbital and plantar pain thresholds were significantly reduced(P<0.001),while the expression levels of P2RY12,Iba-1,CGRP,and c-fos proteins in the TNC tissue,and the number of P2RY12 positive cells and P2RY12 mRNA expression were significantly increased in the model group(P<0.001).Compared with the model group,the periorbital and plantar pain thresholds of the EA,antagonist and medication groups were significantly increased(P<0.001),while the expression levels of P2RY12,Iba-1,CGRP,and c-fos proteins,number of P2RY12 positive neurons,and P2RY12 mRNA expression were apparently decreased in the EA,antagonist,medication and EA+agonist groups(P<0.001,P<0.05).The effect of EA was strikingly superior to that of EA+agonist in increasing periorbital and plantar pain thresholds(P<0.001,P<0.05),lowering the expressions of P2RY12,Iba-1,CGRP and c-fos,the number of P2RY12 positive cells,and P2RY12 mRNA expression(P<0.05,P<0.001),and superior to that of medication in lowering the number of P2RY12 positive cells and P2RY12 mRNA expression(P<0.05,P<0.01).Conclusion EA of the specific points of Shaoyang Meridian can prevent the progression of migraine,and reduce pain,which may be related to its function in down-regulating the expressions of P2RY12 protein and mRNA,Iba-1,and CGRP and c-fos proteins(central sensitization-related indexes)in TNC.关键词
慢性偏头痛/电针/P2Y12受体/少阳经特定穴Key words
Chronic migraine/Electroacupuncture/P2Y12 receptor/Specific acupoints of Shaoyang Meridian引用本文复制引用
邱懿,黄语函,宋雨晖,何雨函,韩文华,何可,付磊,刘未艾..电针少阳经特定穴调控P2Y12受体防治大鼠偏头痛慢性化的机制研究[J].针刺研究,2025,50(9):1064-1071,8.基金项目
湖南省自然科学基金区域联合基金项目(No.2023JJ60337) （No.2023JJ60337）
湖南省卫生健康委员会科研重点指导课题项目(No.C202303079147) （No.C202303079147）
长沙市科学技术局一般项目(No.kq2208198) （No.kq2208198）
长沙市科学技术局指导性科技计划项目(No.kzd22053) （No.kzd22053）
湖南中医药大学研究生创新项目(No.2023CX54) （No.2023CX54）
