疑难病杂志2025，Vol.24Issue(9)：1122-1127,6.DOI:10.3969/j.issn.1671-6450.2025.09.018
过表达PRC1对磷酰胺氮芥诱导后卵巢颗粒细胞增殖与凋亡的影响
Effect of PRC1 overexpression on proliferation and apoptosis in chemotherapy-damaged ovarian granulosa cells
摘要
Abstract
Objective To study the effects of protein regulator of cytokinesis 1(PRC1)overexpression on prolifera-tion and apoptosis in ovarian granulosa cells(GCs)damaged by phosphoramide mustard(PM).Methods From April 2023 to January 2024,conducted experiments in the laboratory of the Medical College of Hunan University of Chinese Medicine.Pri-mary rat GCs were treated with PM to simulate chemotherapy injury.Lentiviral transfection achieved PRC1 overexpression.Groups:OE-NC(empty vector),OE-NC+PM,OE-PRC1,OE-PRC1+PM.CCK-8,EdU staining,flow cytometry,and Western blot assessed proliferation,apoptosis,and protein levels.Results PM reduced cell viability,EdU+cells,Bcl-2,and PRC1 while increasing Bax and Cleaved-Caspase-3(all P＜0.01).PRC1 overexpression reversed these effects(P＜0.01 or P＜0.05)and accelerated S-phase progression.OE-PRC1+PM showed lower apoptosis than OE-NC+PM(P＜0.01).Conclusion PRC1 overexpression promotes proliferation and inhibits apoptosis in chemotherapy-damaged GCs.关键词
卵巢颗粒细胞/化疗损伤/胞质分裂蛋白调节因子1/磷酰胺氮芥/细胞增殖/细胞凋亡Key words
Ovarian granulosa cells/Chemotherapy damage/Protein regulator of cytokinesis 1/Phosphoramide mus-tard/Proliferation/Apoptosis分类
医药卫生引用本文复制引用
徐数理,黄欣怡,曾文卓,孙晓峰..过表达PRC1对磷酰胺氮芥诱导后卵巢颗粒细胞增殖与凋亡的影响[J].疑难病杂志,2025,24(9):1122-1127,6.基金项目
国家自然科学基金(81774362,81303004) （81774362,81303004）
湖南省自然科学基金(2018JJ2290) （2018JJ2290）
长沙市自然科学基金(kq2208187) National Natural Science Foundation of China(81774362,81303004) （kq2208187）
Hunan Provincial Natural Science Foundation(2018JJ2290) （2018JJ2290）
Natural Science Foundation of Changsha City(kq2208187) （kq2208187）
