中国动物检疫2025，Vol.42Issue(9)：41-48,8.DOI:10.3969/j.issn.1005-944X.2025.09.008
时间分辨荧光免疫层析技术在动物疫病快速诊断中的应用进展
Advances in the Application of Time-Resolved Fluorescence Immunochromatographic Assay to Rapid Diagnosis of Animal Diseases
摘要
Abstract
Time-resolved fluorescence immunochromatographic assay(TRFICA),as a highly sensitive detection technique integrating time-resolved fluorescence with immunochromatographic techniques,could enable rapid and precise quantitative detection by eliminating background fluorescence interference through delayed signal acquisition using lanthanide rare-earth element markers to replace traditional colloidal gold.TRFICA,with the advantages of accessibility,lowcost,high specificity,excellent stability and zero pollution,had been widely applied in the fields including diagnosis of animal diseases,food safety,detection of medical biomarkers,etc.,supporting early warning and prevention of animal diseases as a key tool because detection reliability was greatly improved by its superior sensitivity and anti-interference capability.Here,the operation principle of TRFICA and the advances in its application to rapid diagnosis of animal diseases were reviewed,aiming to provide a technical reference for veterinary clinical diagnosis and disease control.关键词
时间分辨荧光/免疫层析/镧系元素/动物疫病诊断Key words
TRFICA/immunochromatography/lanthanide elements/diagnosis of animal diseases分类
农业科技引用本文复制引用
庄金秋,李峰,梅建国,苗立中,谢金文..时间分辨荧光免疫层析技术在动物疫病快速诊断中的应用进展[J].中国动物检疫,2025,42(9):41-48,8.基金项目
山东省生猪产业技术体系滨州综合试验站项目(SDAIT-08-13) （SDAIT-08-13）
