中国骨伤2025，Vol.38Issue(8)：792-799,8.DOI:10.12200/j.issn.1003-0034.20241087
阳虚血瘀型膝骨关节炎患者关节液及血液的基因表达分析
Analysis of gene expression in synovial fluid and blood of patients with knee osteoarthritis of Yang deficiency and blood stasis type
摘要
Abstract
Objective To reveal the molecular basis of knee osteoarthritis(KOA)with Yang deficiency and blood stasis syndrome by analyzing the gene expression profiles in synovial fluid and blood of KO A patients with this syndrome.Methods A total of 80 KO A patients were recruited from October 2022 to June 2024,including 40 cases in the non-Yang deficiency and blood stasis group(27 males and 13 females),with an average age of(61.75±3.45)years old;and 40 cases in the Yang defi-ciency and blood stasis group(22 males and 18 females),with an average age of(62.00±2.76)years old.The levels of body mass index(BMI),high-density lipoprotein(HDL),low-density lipoprotein(LDL),fibrinogen,total cholesterol,and D-dimer were recorded and summarized.Blood and synovial fluid samples from patients were collected for gene expression profile mi-croarray sequencing,and then PCR and immunohistochemistry were used for clinical verification on the patients' synovial fluid and cartilage samples.Results Logistic regression analysis showed that compared with KOA patients with non-Yang deficiency and blood stasis syndrome,those with Yang deficiency and blood stasis syndrome had increased BMI,LDL,fibrinogen,total cholesterol,and D-dimer,and decreased HDL,with a clear correlation between the two groups.There were 562 differential genes in the blood,among which 322 were up-regulated and 240 were down-regulated;755 differential genes were found in the synovial fluid,with 350 up-regulated and 405 down-regulated.KEGG signaling pathway analysis of synovial fluid revealed changes in lipid metabolism-related pathways,including cholesterol metabolism,fatty acid metabolism,and PPARG signaling pathway.Analysis of the involved differential genes identified 6 genes in synovial fluid that were closely related to lipid metabolism,namely LRP1,LPL,ACOT6,TM6SF2,DGKK,and PPARG.Subsequently,PCR and immunohistochemical verifi-cation were performed using synovial fluid and cartilage samples,and the results were consistent with those of microarray se-quencing.Conclusion This study explores the clinical and genomic correlation between traditional Chinese medicine syn-dromes and knee osteoarthritis from the perspective of lipid metabolism,and proves that abnormal lipid metabolism is closely related to KOA with Yang deficiency and blood stasis syndrome from both clinical and basic aspects.关键词
基因芯片/基因表达/膝骨关节炎/中医证型/脂质代谢Key words
Gene chip/Gene expression/Knee osteoarthritis/Traditional Chinese medicine syndrome type/Lipid metabolism分类
医药卫生引用本文复制引用
化昊天,张中意,金钊凯,楼鹏强,孟卓,章安琪,张扬,童培建..阳虚血瘀型膝骨关节炎患者关节液及血液的基因表达分析[J].中国骨伤,2025,38(8):792-799,8.基金项目
浙江省自然科学基金重大项目(编号:LD22C060002) （编号:LD22C060002）
国家自然科学基金(编号:82274547,82474240) （编号:82274547,82474240）
浙江省医药卫生科研项目(编号:2024KY1223)Major Project of Zhejiang Provincial Natural Science Foundation(No.LD22C060002) （编号:2024KY1223）
