应用化学2025，Vol.42Issue(9)：1209-1220,12.DOI:10.19894/j.issn.1000-0518.240400
基于UPLC-Q Exactive Plus-MS技术的清热化毒丸化学成分分析
Chemical Composition Analysis of Qingre Huadu Pill Based on Ultra-Performance Liquid Chromatography Tandem Quadrupole Electrostatic Field Orbit Trap Mass Spectrometery
摘要
Abstract
The chemical composition of Qingrejie Huadu Pill was analysed and identified based on high performance liquid chromatography tandem quadrupole electrostatic field orbit trap mass spectrometry.Use UPLC chromatographic column(2.1 mm×150 mm,2.5 μm),and gradient elution with acetonitrile(A)-0.1%formic acid water(B).The mass spectrometry analysis was performed using an ESI ion source,and the data were collected in positive and negative ion modes.The Xcalibur 4.1 software and the databases such as PubChem,ChemicalBook,and ChemSpider were used to compare the information the reference substance and combine the literature information to analyze the chemical components in Qingre Huadu Pill.A total of 65 chemical constituents were identified,including 5 organic acids,26 flavonoids,11 terpenoids,7 alkaloids,3 amino acids,10 anthraquinones,and 3 other compounds.The UPLC-Q Exactive Plus-MS technique can rapidly identify the chemical constituents in the Qingre Huadu Pill with high accuracy,which provides reliable basis for further research on the material basis of pharmacological effects.关键词
清热化毒丸/高效液相色谱串联四极杆静电场轨道阱质谱/化学成分鉴定/质谱碎裂机理Key words
Qingre Huadu Pill/UPLC-Q Exactive Plus-MS/Chemical composition determination/Mass spectrometry fragmentation mechanism分类
化学化工引用本文复制引用
南敏伦,赵建慧,张瀚水,马洋,马国花,马鑫鑫,赵昱玮,赫玉芳..基于UPLC-Q Exactive Plus-MS技术的清热化毒丸化学成分分析[J].应用化学,2025,42(9):1209-1220,12.基金项目
吉林省科技发展计划项目(No.20220204014YY)资助 Supported by Jilin Province Science and Technology Development Plan Project(No.20220204014YY) （No.20220204014YY）
