中国临床药学杂志2025，Vol.34Issue(8)：614-618,5.DOI:10.19577/j.1007-4406.2025.08.009
UPLC-MS/MS测定桑枝总生物碱片中1-脱氧野尻霉素的含量
Determination of 1-deoxynojirimycin in mulberry twig alkaloids tablets by UPLC-MS/MS
刘亚杰 1靳世英 2徐艳敏2
作者信息
- 1. 邯郸市眼科医院药学部,邯郸 056008
- 2. 邯郸市中心医院药学部,邯郸 056001
- 折叠
摘要
Abstract
AIM A method was developed for the determination of 1-deoxynojirimycin in mulberry twig alkaloids tablets by ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).METHODS The sample solution was separated using a Shimadzu GIST HP AQ C18 column(100 mm×2.1 mm,3 μm),employing acetonitrile with 0.1％formic acid aqueous solution(10:90)as the mobile phase.The column temperature was maintained at 30 ℃ with a flow rate of 0.2 mL·min-1.Electrospray ionization(ESI)positive ions source and multiple-reaction monitoring modes were applied to analyze with the m/z 164.1→69.1 as quantitative ions for quantitative analysis.RESULTS There was a strong linear relationship between the peak areas and the concentration of 1-deoxynojirimycin,ranging from 0.503 to 25.150 μg·mL-1.The correlation coefficient was 0.999 3 and the spiking recovery was 100.66％with a relative standard deviation(RSD)of 2.33％.The RSDs for precision,repeatability and stability were all less than 4.0％.The mass fraction of 1-deoxynojirimycin ranged from 97.92％to 101.86％across 3 batches of samples.CONCLUSION The proposed method has good advantages of operating easily,highly sensitive,analyzing rapidly and good durability of column which can be used for the determination of 1-deoxynojirimycin in mulberry twig alkaloids tablets.关键词
桑枝总生物碱片/1-脱氧野尻霉素/质量评价/超高效液相色谱串联质谱法Key words
mulberry twig alkaloids tablet/1-deoxynojirimycin/quality evaluation/ultra-high performance liquid chromatography-tandem mass spectrometry分类
医药卫生引用本文复制引用
刘亚杰,靳世英,徐艳敏..UPLC-MS/MS测定桑枝总生物碱片中1-脱氧野尻霉素的含量[J].中国临床药学杂志,2025,34(8):614-618,5.
