中国农业科技导报2025，Vol.27Issue(9)：79-91,13.DOI:10.13304/j.nykjdb.2024.0208
紫色辣椒花青素降解相关基因的挖掘与分析
Mining and Analysis of Genes Related to Anthocyanin Degradation in Purple Pepper
摘要
Abstract
To further study the molecular mechanisms of anthocyanin degradation in purple pepper,the transcriptome data of purple unripe peppers at 30 d after anthesis and red ripe fruits at 60 d after anthesis from 3 varieties(HN191,HN192,HN005)were analyzed.The results showed that 7 104 common differentially expressed genes were identified.GO and KEGG enrichment analysis results inidcated that these genes were predominantly associated with processes such as cell wall,secondary metabolism,lipid metabolism,carbohydrate metabolism and amino acid metabolism.Further analysis identified 3 MBW(MYB-bHLH-WD40)transcription factors(including 1 MYB and 2 WRKY),3 peroxidases and 2 β-glucosidase encoding genes.Additionally,a MADS-box transcription factor and a NAC transcription factor were identified through weighted gene co-expression network analysis.The results of qRT-PCR revealed that the expression levels of these genes were higher in red ripe fruits than those in purple unripe fruits,which proposed that these 10 candidate genes might be associated with the degradation of anthocyanins in purple pepper fruits.Above results provided potential candidate genes for the breeding of mature purple peppers.关键词
辣椒/紫色/花青素/降解/加权基因共表达网络分析Key words
chili pepper/purple/anthocyanin/degradation/weighted gene co-expression network analysis分类
农业科技引用本文复制引用
谭华强,李丽平,铁曼曼,杨家勤,郑晓云,潘绍坤,唐有万..紫色辣椒花青素降解相关基因的挖掘与分析[J].中国农业科技导报,2025,27(9):79-91,13.基金项目
四川省"天府学者"特聘专家项目(邓伟2021-58) （邓伟2021-58）
四川省农作物"十四五"育种攻关项目(2021YFYZ0022) （2021YFYZ0022）
四川省自然科学基金面上项目(24NSFSC0872). （24NSFSC0872）
