中国实验动物学报2025，Vol.33Issue(8)：1133-1143,11.DOI:10.3969/j.issn.1005-4847.2025.08.004
高原缺氧环境大鼠深静脉血栓模型的建立与思考
Establishment and assessment of deep vein thrombosis model in rats in a plateau hypoxic environment
摘要
Abstract
Objective To establish a rat model of venous thrombosis in a plateau hypobaric hypoxic environment and to investigate the effect of this environment on venous thrombosis.Methods A total of 144 healthy male SD rats were assigned randomly to four groups(n=36 rats per group):a plains sham operation(A)group,plains operation(B)group,plateau altitude 6000 m+sham operation(C)group,and plateau altitude 6000 m+surgery(D)group.Rats in A and B groups were maintained in a plains normoxic environment,while rats in C and D groups C and D were subjected to a plateau environment.Rats in the surgical groups underwent quantitative constriction to incompletely obstruct the inferior vena cava blood flow.Each group was further divided into subgroups based on time:1,3,5,7,14,and 21 d(n=6 rats per group).Regular vascular ultrasound monitoring was conducted,and blood samples were taken for whole blood viscosity testing and the assessment of inflammatory indicators,including endothelin-1(ET-1),interleukin-6(IL-6)and tissue factor(TF).Coagulation function was evaluated through the activated partial thromboplastin time(APTT),prothrombin time(PT),thrombin time(TT),fibrinogen(FIB)and D-dimer.After the observation period,the experimental animals were sacrificed and the limbs were removed.Thrombus samples were stained with hematoxylin/eosin(HE),and the thrombus wet mass was measured.Results The thrombosis incidence was significantly higher in the plateau D group than in B group,accompanied by a marked increase in blood viscosity and hematocrit(P＜0.01).Additionally,levels of ET-1,IL-6,and TF were significantly elevated(P＜0.05),indicating a coagulation disorder.Conclusions A plateau hypoxic environment model can be successfully simulated by quantitative coarctation of the inferior vena cava,combined with a specialized environmental chamber.The findings of this study suggest that a plateau hypoxic environment promotes venous thrombosis.关键词
高原/静脉血栓/动物模型/大鼠Key words
high altitude/venous thrombosis/animal model/rat分类
生物科学引用本文复制引用
韩小博,杨曦,敖其,张雷,韩红伟,刘智学,柴雅豪,高家威,邓鑫凯,李啸,吴家林,韩小丽,李国祥,赵胤杰..高原缺氧环境大鼠深静脉血栓模型的建立与思考[J].中国实验动物学报,2025,33(8):1133-1143,11.基金项目
新疆军区总医院拔尖项目(2022BJ003),新疆天山英才医药卫生高层次人才培养计划(TSYC202301B046).Funded by Top Project of General Hospital of Xinjiang Military Command(2022BJ003),Tianshan Talent Training Program for High-Level Talent in Medicine and Health(TSYC202301B046). （2022BJ003）
