中国实验动物学报2025，Vol.33Issue(8)：1144-1152,9.DOI:10.3969/j.issn.1005-4847.2025.08.005
不同性别CUMS模型大鼠的肠道菌群差异与抑郁症发病关系
Relationship between gut microbiota and onset of depression in chronic unpredictable mild stress model rats of both sexes
摘要
Abstract
Objective To observe the differences in gut microbiota in chronic unpredictable mild stress(CUMS)-induced depression model rats of both sexes,and to provide experimental evidence for exploring sex differences in depression onset.Methods Thirty-two healthy SD rats were divided randomly into four groups based on sex:Male control group(Control-M),Female control group(Control-F),Male model group(Model-M),and Female model group(Model-F)(n=8 rats per group).Rats in the control groups were fed without stimulation,while rats in the model groups were stimulated using the 28 d CUMS-induced depression method.After successful modeling,fresh feces were collected from all rats for high-throughput 16S rRNA sequencing.Behavioral observations were also conducted before and after preparing the model.Results The result of sucrose-preference,open-field,and forced-swimming tests differed significantly between the control and model groups.The result of the sucrose-preference test also differed between the sexes,while there was no difference in the open-field or forced-swimming test between the sexes.The α and β diversity of the gut microbiota genera showed an upward trend in the CUMS group compared with the control group.The ratio of Firmicutes/Bacteroidetes and the richness of the Roseburia and Lachnospiraceae_NK4A136_group were decreased in male rats but showed an increasing trend in female rats.Conclusions The ratio of Firmicutes/Bacteroidetes in the gut microbiota may be a key factor affecting the difference in the onset of depression between males and females,while the Roseburia and Lachnospiraceae_NK4A136_group be potential factors in correcting the gut microbiota and improving the symptoms of depression.关键词
抑郁症/CUMS/肠道菌群/性别差异Key words
depression/chronic unpredictable mild stress/gut microbiota/sex difference分类
生物科学引用本文复制引用
朱抗洪,高雨萌,黄梦雪,杨柳,高子瞻,储浩,邓楠,胡玲,吴子建..不同性别CUMS模型大鼠的肠道菌群差异与抑郁症发病关系[J].中国实验动物学报,2025,33(8):1144-1152,9.基金项目
安徽省自然科学基金(2108085MH307),安徽中医药大学校级探索性项目(AHUCM2024TS020).Funded by Anhui Provincial Natural Science Foundation(2108085MH307),Exploratory Project at Anhui University of Chinese Medicine(AHUCM2024TS020). （2108085MH307）
