中国农业科技导报2025，Vol.27Issue(9)：240-249,10.DOI:10.13304/j.nykjdb.2025.0210
酵母钴的制备、表征及其在鱼抗应激中的应用
Preparation and Characterization of Yeast Cobalt and Its Application in Fish Stress Resistance
摘要
Abstract
Cobalt(Co)is an important essential trace element for aquatic animals and is critical for maintaining their physiological and metabolic functions.Probiotics can convert inorganic cobalt into organic cobalt forms with less toxicity and higher bioavailability,providing dual benefits of cobalt and probiotics.Yeast R was screened to improve cobalt-enriched ability from the probiotics recommended by the European Union Qualification for the Safety of Microbial Strains(QPS),which had high tolerance and enrichment capacity for cobalt chloride.The optimal cobalt enrichment conditions was determined as initial pH 5.5,incubation temperature 30℃,incubation time 76 h,and the addition of 1.5 g·L-1 cobalt chloride for 4 h,resulting in total cobalt content of 3.78 g·kg-1.Characterization tools confirmed the possible presence of spherical cobalt on the surface of cobalt-enriched yeast(R-Co)nanoparticles.In the zebrafish test,the addition of 0.166 7 mg·kg-1 R-Co had no effect on growth performance and improved survival rate under ammonia-nitrogen stress and cold stress compared with the addition of 0.166 7 mg·kg-1 inorganic cobalt,suggesting that yeast R is an efficient cobalt-enriched strain with the potential of replacing inorganic cobalt for promising applications in improving the health and resilience of aquatic animals.关键词
益生酵母/微量元素钴/表征/斑马鱼/抗应激Key words
probiotic yeast/trace element cobalt/characterization/zebrafish/stress resistance分类
农业科技引用本文复制引用
石柯柔,周志刚,王楠,栾银银,李彦卿,张震,药园园,冉超,丁倩雯,杨雅麟..酵母钴的制备、表征及其在鱼抗应激中的应用[J].中国农业科技导报,2025,27(9):240-249,10.基金项目
国家重点研发计划项目(2022YFC2105004) （2022YFC2105004）
国家自然科学基金项目(32172958,U21A20267,32330110 和 32172991) （32172958,U21A20267,32330110 和 32172991）
中国农业科学院农业科技创新工程项目(CAAS-ZDRW202305,CAAS-ASTIP-2023-IFR-05) （CAAS-ZDRW202305,CAAS-ASTIP-2023-IFR-05）
农业农村部富硒产品开发与质量控制重点实验室安康富硒渔业专家工作站项目. （）
