中国输血杂志2025，Vol.38Issue(9)：1154-1161,8.DOI:10.13303/j.cjbt.issn.1004-549x.2025.09.005
人诱导多能干细胞来源血小板外泌体与单采血小板外泌体特征及功能差异性研究
Comparative analysis of characteristics and functions of exosomes from human induced pluripotent stem cell-de-rived platelets and apheresis platelets
摘要
Abstract
Objective To compare the biological characteristics of human induced pluripotent stem cell-derived platelet exosomes(hiPSC-Plt-Exos)with those of conventional apheresis platelet exosomes(Plt-Exos),specifically focusing on their differential abilities to enhance the proliferation and migration of human umbilical cord mesenchymal stem cells(hUC-MSCs).Methods Exosomes were isolated from hiPSC-derived Plt and apheresis Plt concentrate using size exclusion chro-matography.These exosomes were then characterized through nanoparticle tracking analysis(NTA),transmission electron microscopy(TEM),and Western blotting.Co-culture experiments into hUC-MSCs were conducted with hiPSC-Plt-Exos and apheresis Plt-Exos,respectively.Their effects on the proliferation and migration of hUC-MSCs were assessed via cell prolif-eration assays and scratch tests.Results hiPSC-Plt-Exos and apheresis Plt-Exos exhibited comparable particle sizes,mor-phological features(such as the characteristic cup-shaped structure),and surface markers(including CD9 and HSP70).Notably,hiPSC-Plt-Exos demonstrated a significantly greater ability to enhance the proliferation and migration of hUC-MSCs compared to apheresis Plt-Exos(P＜0.05).These differences provide critical comparative data for their application in vari-ous clinical contexts.Conclusion This study establishes a theoretical foundation for developing precise therapeutic strate-gies based on hiPSC-Plt-Exos.Furthermore,it underscores the necessity of selecting the appropriate type of exosomes ac-cording to the specific disease microenvironment to achieve optimal therapeutic outcomes.关键词
人诱导多能干细胞/单采/血小板/外泌体/人脐带间充质干细胞Key words
human induced pluripotent stem cells/apheresis/platelets/exosomes/human umbilical cord mesenchy-mal stem cells分类
医药卫生引用本文复制引用
黄韦华,顾海慧,查占山,臧艳,秦爱华,冯子阳,唐河山,郭菲,吴春燕,沈秋,钱宝华..人诱导多能干细胞来源血小板外泌体与单采血小板外泌体特征及功能差异性研究[J].中国输血杂志,2025,38(9):1154-1161,8.基金项目
国家自然科学基金项目(81970165、82200257) （81970165、82200257）
海军军医大学第一附属医院固海计划项目(GH-145-37) （GH-145-37）
