中国循证儿科杂志2025，Vol.20Issue(4)：285-293,9.DOI:10.3969/j.issn.1673-5501.2025.04.008
晚发型脓毒症新生儿外周血免疫细胞亚群变化的病例对照研究
Changes in immune cell subsets in neonates with late-onset sepsis:A case-control study
韩俊彦 1李淑涓 1陶成武 2王静 2周玉峰 3杨毅 3张晓明 2曹云4
作者信息
- 1. 国家儿童医学中心,复旦大学附属儿科医院,新生儿诊疗中心 上海,201102
- 2. 中国科学院上海免疫与感染研究所 上海,200031
- 3. 国家卫生健康委员会新生儿疾病重点实验室(复旦大学)上海,201102
- 4. 国家儿童医学中心,复旦大学附属儿科医院,新生儿诊疗中心 上海,201102||国家卫生健康委员会新生儿疾病重点实验室(复旦大学)上海,201102
- 折叠
摘要
Abstract
Background Sepsis is a significant cause of morbidity and mortality in neonates.Immune system plays a crucial role in the development and recovery of sepsis.However,evidence on the changes of immune cells following neonatal sepsis onset is limited.Objective To describe the changes of immune cell subsets in neonates with late-onset sepsis and to explore the correla-tion between the distribution of immune cell subsets and short-term prognosis.Design A case-control study.Methods Neonates with late-onset sepsis(including both preterm and full-term infants)were enrolled as the sepsis group,and matched neonates with-out infectious diseases were included as the control group.Flow cytometry was used to detect the proportions and absolute counts of immune cell subsets,including polymorphonuclear cells(PMN),peripheral blood mononuclear cells(PBMC),natural killer cells(NK),monocytes(Mo),and T/B lymphocytes.Main outcome measures The distribution of immune cell subsets between the case and control groups during the onset and recovery phases of sepsis were compared.And the relationships between subset distri-bution and clinical outcomes were investigated.Results At the onset of sepsis,the proportions of PMN cells and CD14+/CD16+monocytes in the peripheral blood of sepsis neonates,especially preterm infants,were significantly higher than those in the control group.In contrast,CD3+T lymphocytes,particularly CD3+/CD4+T lymphocytes,were significantly lower than those in the control group.During the recovery period of sepsis,the levels of NK cells and T lymphocytes(including CD3+/CD4+T cells and CD3+/CD8+T cells)increased significantly,with a more pronounced upward trend in full-term infants.Multivariate regression analysis showed that reduced proportions of CD3+T cells(aOR=-0.11,95%CI:-0.23,-0.01,P=0.044)and CD3+/CD4+T cells(aOR=-0.16,95%CI:-0.32,-0.01,P=0.040)were associated with severe sepsis.Conclusion The changes in immune cell subsets differ between preterm and full-term infants during the sepsis onset.A reduced proportion of T lymphocytes is associated with severe sepsis.关键词
新生儿/脓毒症/免疫细胞/流式细胞术Key words
Neonates/Sepsis/Immune cell/Flow cytometry引用本文复制引用
韩俊彦,李淑涓,陶成武,王静,周玉峰,杨毅,张晓明,曹云..晚发型脓毒症新生儿外周血免疫细胞亚群变化的病例对照研究[J].中国循证儿科杂志,2025,20(4):285-293,9.
