中国医学前沿杂志（电子版）2025，Vol.17Issue(7)：48-55,8.DOI:10.12037/YXQY.2025.07-07
腹侧被盖区多巴胺神经元促肾上腺皮质激素释放激素受体1参与焦虑对咬合干扰致口颌面痛的促进作用
CRHR1 of ventral tegmental dopamine neurons participates in the facilitation of anxiety on occlusal interference induced orofacial pain
摘要
Abstract
Objective To investigate the role of the corticotropin releasing hormone receptor 1(CRHR1)of dopamine(DA)neurons of ventral tegmental area(VTA)in experimental occlusion interference(EOI)induced orofacial pain.Methods Anxiety levels of 115 Sprague-Dawley(SD)rats were assessed using the elevated plus maze test to establish an anxiety spectrum,followed by K-means cluster analysis to develop classification criteria.High-anxiety(HA)and low-anxiety(LA)rats were selected for EOI modeling.The development of orofacial pain was evaluated by measuring the mechanical head withdrawal threshold(HWT)in masticatory muscles(7 rats per group).Immunofluorescence staining was performed to examine differences in CRHR1 expression in VTA DA neurons at 6 days post-EOI(6 rats per group).After confirming HA rats'heightened sensitivity to EOI,in vivo electrophysiology was conducted on HA rats to assess the effects of CRHR1 activation in VTA DA neurons on(1)neuronal firing frequency(10 neurons from 5 rats per group),and(2)VTA local field potentials(14 channels from 5-7 rats per group)at 6 days post-EOI.Subsequent HWT measurements were performed following CRHR1 activation in VTA DA neurons(7 rats per group).Results The anxiety levels of SD rats could be classified into three significantly different categories HA,moderate anxiety(MA),and LA(P<0.001).HA rats showed faster development of orofacial pain following EOI(P<0.01),and lower expression of CRHR1 in VTA DA neurons(P<0.001).Activation of CRHR1 in VTA DA neurons significantly increased neuronal firing rates(P<0.05),partially altered the local field potential of VTA region(P<0.05),and significantly decreased HWT values(P<0.05).Conclusions CRHR1 of VTA DA neurons participates in the facilitative effect of anxiety on EOI induced orofacial pain.关键词
咬合干扰/口颌面痛/腹侧被盖区/多巴胺神经元/促肾上腺皮质激素释放激素受体1Key words
Occlusal interference/Orofacial pain/Ventral tegmental area/Dopamine neurons/Corticotropin releasing hormone receptor 1引用本文复制引用
张垚君,李源,莫思怡,刘婧雯,徐啸翔,曹烨..腹侧被盖区多巴胺神经元促肾上腺皮质激素释放激素受体1参与焦虑对咬合干扰致口颌面痛的促进作用[J].中国医学前沿杂志（电子版）,2025,17(7):48-55,8.基金项目
国家自然科学基金(82170982) （82170982）
北京市自然科学基金(7242171,7244443) National Natural Science Foundation of China(82170982) （7242171,7244443）
Beijing Natural Science Foundation(7242171,7244443) （7242171,7244443）
